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$380.00
Sku: GMP-TL644-0050
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by July 29, 2026
Description
The recombinant human PDGF-BB-His fusion protein is a high-purity homodimeric growth factor produced in HEK-293 expression systems, featuring a C-terminal polyhistidine tag for nickel-affinity chromatography purification.
As a core member of the PDGF/VEGF superfamily, PDGF-BB specifically engages PDGFR-β to activate Ras-MAPK and PI3K-Akt signaling pathways, driving directed migration, proliferation, and differentiation of mesenchymal stem cells, pericytes, and vascular smooth muscle cells while enhancing extracellular matrix adhesion through integrin αvβ3 upregulation.
In cell therapy applications, this protein serves as a critical component in 3D bioscaffold culture systems, where controlled release kinetics enhance ex vivo expansion and paracrine activation of mesenchymal stem cells (MSCs), synergize with VEGF to engineer functional vascular networks, and promote neural progenitor differentiation into oligodendrocyte precursor cells (OPCs), thereby creating vascularized microenvironments for tissue-engineered grafts.
The native disulfide-bonded dimeric configuration preserves full bioactivity, while the His-tag design enables precise purification without Fc-mediated off-target activation, making it particularly suitable for regenerative medicine applications requiring spatiotemporal control of growth factor concentrations.
Specifications
| Specification | Details |
|---|---|
| Synonyms | Platelet-derived growth factor subunit B, PDGF-2, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis |
| Accession | UniProt P01127 |
| Expressed Region | Ser82–Thr190 |
| Tag | C-terminal 6×His-tag |
| Expression System | HEK293 cells |
| Predicted Molecular Weight | 13.1 kDa |
| Purity | > 90% as determined by SDS-PAGE |
| Endotoxin | < 0.1 EU per 1 μg of protein (LAL method) |
| Formulation | Lyophilized from sterile 20 mM phosphate-buffered saline (PBS), pH 7.4, typically containing 6–8% (w/v) mannitol as a stabilizing protectant |
Features
-
✔
Native Dimeric PDGF-BB Activity
Preserves disulfide-bonded homodimer structure for authentic PDGFR-β signaling.
-
✔
Potent MSC-Targeted Signaling
Activates Ras-MAPK and PI3K-Akt pathways to drive migration, proliferation, and differentiation of mesenchymal cells.
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✔
High Purity, Low Endotoxin
HEK293 expression and His-tag purification ensure consistent quality for sensitive cell culture systems.
-
✔
Excellent Batch Stability
Reliable performance across batches supports reproducible tissue engineering workflows.
Applications
Mesenchymal Stem Cell (MSC) Expansion
Enhances MSC proliferation and paracrine activity in 2D and 3D culture systems.
Vascularized Tissue Engineering
Synergizes with VEGF to support pericyte recruitment and functional vascular network formation.
3D Bioscaffold-Based Cell Culture
Enables controlled growth factor delivery for spatially organized tissue constructs.
Neural & Regenerative Medicine Research
Supports neural progenitor differentiation and regeneration-oriented microenvironment design.
Stability & Storage
- √ Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored inthe original sealed container under desiccant.
- √ Reconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4)maintaining final concentration ≥100 µg/mL to prevent adsorption.
- √ Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Document
FAQ
What is Recombinant Human PDGF-BB Protein?
Recombinant Human PDGF-BB Protein is a recombinant form of human platelet-derived growth factor BB. It is used for research applications involving PDGF-related signaling, including studies of cellular proliferation, migration, and differentiation.
How is Recombinant Human PDGF-BB Protein produced?
Recombinant Human PDGF-BB Protein is produced using a HEK293 cell expression system. The protein contains the human PDGF-BB expressed region Ser82-Thr190 and a C-terminal 6×His tag.
What applications can Recombinant Human PDGF-BB Protein be used for?
Recombinant Human PDGF-BB Protein can be used for research applications involving mesenchymal stem cell studies, cell signaling research, tissue engineering research, and growth factor related cell culture workflows.
How does PDGF-BB Protein support cell signaling research?
PDGF-BB Protein is studied for its interaction with PDGFR-β and related signaling pathways involved in cellular migration, proliferation, and differentiation of mesenchymal cells, pericytes, and vascular smooth muscle cells.
What quality controls are performed for this PDGF-BB Protein?
Quality testing includes appearance, identity, visible particle inspection, moisture analysis, pH testing, purity evaluation by SDS-PAGE, endotoxin testing, mycoplasma testing, and sterility testing.
The product specification includes purity greater than 90% by SDS-PAGE and endotoxin below 0.1 EU/μg.
What expression system is used for Recombinant Human PDGF-BB Protein?
Recombinant Human PDGF-BB Protein is produced using HEK293 cells as the expression system.
Does Recombinant Human PDGF-BB Protein contain a His-tag?
Yes. The protein contains a C-terminal 6×His tag designed to support recombinant protein purification and analytical workflows.
How should Recombinant Human PDGF-BB Protein be stored?
The lyophilized protein is stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
How should Recombinant Human PDGF-BB Protein be reconstituted?
The protein can be reconstituted using sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4). A final concentration of ≥100 µg/mL is recommended during reconstitution.
Is Recombinant Human PDGF-BB Protein intended for therapeutic use?
No. This product is supplied for Research Use Only (RUO). It is not intended for human use, animal therapeutic applications, diagnostic use, or clinical treatment.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
