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$192.00
Sku: Y03301-50ug
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 20, 2026
Description
Noggin, a secreted protein belonging to the TGF-β superfamily, functions as an endogenous antagonist of bone morphogenetic proteins (BMPs) and plays a key regulatory role in embryonic development, particularly in neural patterning and bone formation. By inhibiting BMP signaling—especially that of BMP4, which normally suppresses neural differentiation—Noggin promotes the differentiation of stem cells (such as embryonic stem cells or induced pluripotent stem cells) into neural precursor cells or neurons. It is therefore commonly used in neural differentiation media for stem cells. Under certain culture conditions, Noggin can also act synergistically with other factors (e.g., FGF2, TGF-β) to help maintain stem cell self-renewal and prevent spontaneous differentiation. Additionally, Noggin is frequently employed in cerebral or neural organoid culture systems to facilitate the formation of neuroepithelial structures.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y03301 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Determined by its abitity to inhibit 5.0 ng/mL of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The ED50 for this effect is 2.0-3.0 ng/mL of Noggin. |
| Accession | Q13253 |
| Alternative Names | Noggin; NOG |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 23 kDa |
| Expression Region | Gln28-Cys232 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Stem Cell Research
Inhibits BMP signaling to maintain pluripotency and direct differentiation in stem cell cultures.
Cell Biology
Regulates morphogenesis and differentiation by antagonizing BMP pathways.
Data Figures
Figure 1: Recombinant Human Noggin (Cat. No. Y03301) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with Y03301 SDS-PAGE.
Figure 2: Measured by its ability to inhibit 5.0 ng/mL of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrocenic cells. The bioactivity of Recombinant Human Noggin (Cat. No.Y03301) is equivalent to competitor.
Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human Noggin (Cat. No. Y03301).
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
