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Sku: GMP-TL506-50
Categories: Protein

Product Information

Construction A DNA sequence encoding the human IL-7(P13232-1:D26-H177)was expressed with a Fc-tag at the C-terminus
Expression Host CHO cells
QC Testing Purity > 95 % as determined by SDS-PAGE and HPLC
Activity Cell proliferation assay was performed on PBMC cells activated with CD3 monoclonal antibody, with an ED50 of 2-20 ng/mL and a corresponding specific activity of > 1× 109 Units/mg (calibrated according to human IL-7 reference standard (NIBSC code: 90/530))
Endotoxin < 0.01 EU per 1μg of the protein by the LAL method
Molecular Mass The recombinant human IL-7 protein predicts a molecular mass of 43.8 kD
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization
Stability & Storage 24 months at 2℃ to 8℃ in lyophilized state.
6 months at -20℃ under sterile conditions after reconstitution.
12 months at -80℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100 μg/mL.
Avoid repeated freeze-thaw cycles.


Background

IL-7 is an important cytokine for the growth, survival, and differentiation of T, B, and NK cells. The heterodimer formed by IL-7 and hepatocyte growth factor (HGF) is a precursor B cell growth stimulating factor. Studies on gene knockout in mice have shown that IL-7 plays a crucial role in the survival of lymphocytes. IL-7 stimulates pluripotent stem cells to differentiate into lymphoid progenitor cells.

References

  1. Aliyari Z, Alemi F, Brazvan B, Tayefi Nasrabadi H, Nozad Charoudeh H. CD26+ Cord Blood Mononuclear Cells Significantly Produce B, T, and NK Cells. Iran J Immunol. 2015;12(1):16–26.

  2. H.R. Kim, K.A. Hwang, S.H. Park, I. Kang.IL-7 and IL-15: biology and roles in T-Cell immunity in health and disease.Crit Rev Immunol, 28 (2008), pp. 325-339.

  3. Su N, Shi SX, Zhu X, Borazanci A, Shi F, Gan Y. Interleukin-7 expression and its effect on natural killer cells in patients with multiple sclerosis. J Neuroimmunol (2014) 276:180–6.

Document

Recombinant Human IL-7 Protein

Recombinant Human IL-7 Protein_SDS

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