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Description
IL-7 is an important cytokine for the growth, survival, and differentiation of T, B, and NK cells. The heterodimer formed by IL-7 and hepatocyte growth factor (HGF) is a precursor B cell growth stimulating factor. Studies on gene knockout in mice have shown that IL-7 plays a crucial role in the survival of lymphocytes. IL-7 stimulates pluripotent stem cells to differentiate into lymphoid progenitor cells.
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Specifications
| Cytokines | |
| Expression Host | HEK293 cells |
| Synonym | MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor |
| Protein Sequence | A DNA sequence encoding the human IL-3 (GenBank: AAA59146.1) was expressed with a His-tag at the C-terminus. |
| Molecular Mass | The recombinant human IL-3 protein consists of 139 amino acids and predicts a molecular mass of 15.9 kD. |
| QC Testing Purity | > 90% as determined by SDS-PAGE. |
| Endotoxin | < 0.1EU per 1 μg of the protein by the LAL method. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6% mannitol are added as protectants before lyophilization. |
| Stability | Lyophilized preparation can be stored at -20℃. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. |
| Storage | Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100μg/mL. Avoid repeated freeze-thaw cycles. |
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