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$260.00
Sku: GMP-TL512-0050
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by April 09, 2026
Description
The recombinant human IL-6- protein is produced in HEK-293 cells via transient transfection, featuring a C-terminal polyhistidine (His) tag for nickel-affinity purification.
As a pleiotropic α-helical cytokine, IL-6 activates JAK-STAT and MAPK signaling pathways by binding to the IL-6Rα/gp130 heterodimeric receptor, regulating immune responses, hematopoiesis, and cellular differentiation.
In cell and gene therapy applications, it critically enables: ex vivo expansion of hematopoietic stem cells (HSCs) and T cells, enhancement of effector functions in NK and CAR-T cells, directed differentiation of induced pluripotent stem cells (iPSCs) toward hematopoietic lineages, and synergistic construction of vascularized tissue-engineered microenvironments.
The native bioactive conformation is preserved, while the His-tag design ensures precise purification without Fc-mediated off-target effects, making it particularly suitable for regenerative medicine and cell therapy systems requiring spatiotemporal control of cytokine concentrations.
Specifications
| Cytokines Details | |
|---|---|
| Synonyms | IL6, Interleukin-6, BSF2, HSF, IFNB2 |
| Accession | UniProt P05231 |
| Expressed Region | Val30–Met212 |
| Tag | C-terminal 6×His-tag |
| Expression System | HEK293 cells |
| Molecular Weight | 21.6 kDa |
| Purity | > 95% as determined by SDS-PAGE |
| Endotoxin | < 0.1 EU per 1 μg of protein (LAL method) |
| Biological Activity | Measured by TF-1 cell proliferation assay, corresponding to a specific activity of ≥ 5 × 107 IU/mg |
| Formulation | Lyophilized from sterile PBS (pH 7.4), typically supplemented with 6% mannitol as a stabilizing protectant |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%, ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expected biological responses in target cells, suitable for a wide range of research applications.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure high consistency between batches, reducing variability in experimental outcomes.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements and broadening its application scope in research.
Applications
Immunology
Study the role of IL-6 in immune responses, inflammation, and autoimmune diseases, providing insights into potential therapeutic targets.
Oncology
Investigate the effects of IL-6 on tumor cells, tumor microenvironment, and cancer therapy, aiding in the development of novel anti-cancer strategies.
Hematopoiesis
Explore the role of IL-6 in the regulation of blood cell production and its implications for treating hematological disorders.
Cell Biology
Research the impact of IL-6 on cell signaling pathways, apoptosis, and cellular stress responses, contributing to a deeper understanding of cell biology.
Data Figures
Stability & Storage
- Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration≥100µg/mL to prevent adsorption.
- Handling: Aliquot to avoid repeated freeze-thaw cycles.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
