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$400.00
Sku: TL-301-0100
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
As a master regulator of Th2 immunity, IL-4 activates the JAK1/3-STAT6 signaling cascade via the IL-4Rα/γc receptor complex, directing naive CD4+ T-cell differentiation into Th2 effectors secreting IL-5/IL-13 while mediating B-cell class switching to IgE/IgG1 and FcεRI upregulation.
The recombinant human IL-4-His fusion protein is a high-purity functional cytokine produced in HEK-293 expression systems, featuring a C-terminal hexahistidine tag to facilitate affinity purification and structural integrity.
In cell therapy applications, this protein serves as an essential inducer for monocyte-derived immature dendritic cell (imDC) generation through GM-CSF synergy, effectively suppressing CD14 expression and enhancing antigen-capturing capacity to standardize tumor vaccine production.
It also modulates CAR-T cell manufacturing by balancing Th1/Th2 polarization for optimized solid tumor responses and prolongs mast cell survival in vitro to enhance stability of allergen-specific immunotherapies.
The HEK-293-derived expression ensures authentic glycosylation patterns, while the His-tag maintains native conformation and enables high-resolution chromatographic purification.
Specifications
| Specification | Acceptance Criteria | Results |
|---|---|---|
| Appearance | Freeze-dried preparation, the form should be white loose body | Compliance |
| Identity | The electrophoretic bands should be consistent with the molecular weight of the product | Compliance |
| Visible Particle | Should be no visible particle | Compliance |
| Moisture | ≤4.0% | 1.0% |
| pH | 6.5~7.5 | 7.2 |
| Purity | >90.0% | Compliance |
| Endotoxin | <0.1EU/μg | <0.1EU/μg |
| Mycoplasma | Should be negative | Negative |
| Sterility | No growth | No growth |
| Biological Activity | Cell proliferation assay was performed with TF-1 cells, and the activity ≥ 1×106 IU/mg | 7.9×106 IU/mg |
Features
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✔
High Biological Potency (TF-1 Validated)
Demonstrates strong proliferative activity in TF-1 cell assays (7.9 × 106 IU/mg), ensuring reliable Th2-skewing and DC induction performance.
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✔
HEK293 Expression with Authentic Glycosylation
Mammalian expression preserves native folding and glycosylation, supporting consistent IL-4Rα/γc signaling activation.
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✔
Low Endotoxin, Cell-Therapy Compatible
Endotoxin level < 0.1 EU/µg and mycoplasma-negative, suitable for immune cell culture and CGT research workflows.
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✔
Batch-to-Batch Consistency
Manufactured under controlled QC standards with verified purity (>90% SDS-PAGE), minimizing experimental variability.
Applications
Monocyte-Derived Dendritic Cell (imDC) Generation
Used synergistically with GM-CSF to suppress CD14 expression and drive standardized immature DC differentiation.
T-Cell Polarization Studies
Directs naïve CD4⁺ T cells toward Th2 lineage via STAT6 activation, supporting immune-profiling research.
CAR-T / CGT Process Development
Modulates Th1/Th2 balance during T-cell manufacturing to optimize functional phenotype for solid tumor research.
Mast Cell & Allergy Research Models
Prolongs mast cell survival in vitro, enabling stable allergen-response and IgE-related studies.
Stability & Storage
- Lyophilized powder: Store at −20 °C in the original sealed container.
- Reconstitution: Reconstitute with sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4) prior to use.
- Handling: Aliquot after reconstitution and avoid repeated freeze–thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.
• Storage: Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
