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$300.00
Sku: GMP-TL508-0050
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by March 15, 2026
Description
The recombinant human IL-12 fusion protein is a heterodimer produced by transient transfection in HEK-293 cells, consisting of an IL-12A (p35) subunit with a 6×his-tag and an IL-12B (p40) subunit with an Fc-tag.
As a pivotal immunoregulatory cytokine, IL-12 drives naive T cell differentiation into Th1 cells and potently activates T cells and NK cells, stimulating IFN-γ and TNF-α production.
Critically for cell and gene therapy, this protein is essential: it enables ex vivo expansion and activation of CAR-T cells and NK cells, enhancing their anti-tumor cytotoxicity; it is applied in immunotherapies against tumors, metastatic cancers, viral infections (e.g., AIDS), and as an adjuvant, functioning by eliciting potent Th1 immune responses and cytotoxicity; its anti-angiogenic activity also holds therapeutic potential.
The native dimeric structure ensures full bioactivity, while the dual His/Fc-tag design facilitates efficient purification and extends serum half-life, making it an ideal tool for immunotherapy applications.
Specifications
| Cytokines Details | |
|---|---|
| Synonyms | IL-12, p70, Interleukin-12 |
| Accession | UniProt P29459-1 & P29460-1 |
| Expressed Region | Arg23–Ser219 (P29459-1) & Ile23–Ser328 (P29460-1) |
| Tag | His Tag & Fc Tag |
| Expression System | HEK293 cells |
| Predicted Molecular Weight | ~65 kDa |
| Purity | > 90% as determined by SDS-PAGE |
| Endotoxin | < 0.1 EU per 1 μg of protein (LAL method) |
| Biological Activity | Measured by PHA-activated PBMC proliferation assay, corresponding to a specific activity of ≥ 1.5 × 106 IU/mg |
| Formulation | Lyophilized from sterile PBS (pH 7.4), typically supplemented with 6% mannitol as a stabilizing protectant |
Features
-
✔
High Purity: Purified using advanced chromatographic techniques, this product achieves a purity level of over 95%, ensuring accurate and reproducible experimental results.
-
✔
High Biological Activity: Validated through multiple activity assays, this IL-12 protein effectively promotes T cell differentiation and NK cell activity, making it suitable for a range of research applications.
-
✔
Batch Consistency: Produced under stringent quality control measures to ensure high consistency between batches, reducing variability and enhancing experimental reliability.
- ✔ Animal-Free: Manufactured without the use of animal-derived components, adhering to animal-free standards and broadening its applicability for diverse research needs.
Applications
Immunology Research
Study the role of IL-12 in the regulation of immune responses, including the differentiation of T cells and the activation of NK cells, providing insights into immune mechanisms and potential therapeutic targets.
Oncology
Explore the effects of IL-12 on tumor immunity, including its ability to enhance the cytotoxic activity of immune cells against cancer cells, contributing to the development of immunotherapeutic approaches.
Infectious Diseases
Investigate the role of IL-12 in host defense mechanisms against infections, offering valuable information for the development of new treatments for infectious diseases.
Vaccine Development
Utilize IL-12 in the design and testing of vaccines to enhance immune responses and improve vaccine efficacy.
Data Figures
Stability & Storage
- Lyophilized powder: Stable for 12 months at -80°C or 6 months at -20°C when stored in the original sealed container under desiccant.
- Reconstitution: Dissolve in sterile water for injection, 0.9% NaCl, or PBS (pH7.4), maintaining a final concentration ≥ 100 µg/mL to prevent adsorption.
- Handling: Aliquot to avoid repeated freeze-thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
