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$432.00
Sku: Y04001-50ug
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 20, 2026
Description
IL-12 (Interleukin-12) is a key pro-inflammatory cytokine and a member of the IL-12 family, primarily produced by antigen-presenting cells such as dendritic cells, macrophages, and B cells. It is a heterodimeric protein (p70) composed of two subunits, p35 and p40, linked by a disulfide bond. IL-12 promotes Th1 cell differentiation by activating the STAT4 signaling pathway, driving the commitment of naïve CD4⁺ T cells into Th1 cells and stimulating the secretion of pro-inflammatory cytokines such as IFN-γ. It also enhances the activity of NK and T cells by promoting the proliferation and cytotoxic function of NK cells and CTLs (cytotoxic T lymphocytes). Furthermore, IL-12 suppresses Th2 responses by inhibiting IL-4-mediated Th2 cell differentiation, thereby reinforcing cell-mediated immunity.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y00401 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL. |
| Accession | P29460(IL12P40), P29459(IL12P35) |
| Alternative Names | Interleukin-12 subunit alpha; IL-12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell; IL12A ; NKSF1 stimulatory factor chain 1 |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 59 kDa |
| Expression Region | Ile23-Ser328&Arg23-Ser219 |
| Tag Info | Tag free |
| Form | Lyophilized |
| Buffer | PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Hematology
Influences maturation of NK and T cell functions.
Immunology
Induces Th1 differentiation and enhances cytotoxic immunity.
Cell Biology
Enhances cytotoxicity-related signaling and effector functions.
Data Figures
Figure 1: Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
