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$25.37
Sku: Y04501-10ug
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by May 06, 2026
Description
Recombinant Human Holo-Transferrin (HOLO) is an iron-saturated form of transferrin, a key glycoprotein responsible for iron transport and delivery in biological systems. Unlike apo-transferrin, holo-transferrin is fully loaded with iron, enabling efficient cellular iron uptake via transferrin receptor-mediated endocytosis. It plays a critical role in supporting cell proliferation, metabolism, and viability, especially in serum-free and chemically defined culture systems.
This recombinant protein is expressed in CHO cells and corresponds to the full-length human transferrin sequence (Val20–Pro698), ensuring proper folding and biological functionality. With high purity (≥95%) and low endotoxin levels (≤0.5 EU/mg), this product provides consistent performance in sensitive cell culture applications. Supplied in a tag-free, lyophilized form, it is ideal for large-scale cell culture, bioprocessing, and research use.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y04501 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤0.5 EU/mg by the LAL method. |
| Bioactivity | Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives. |
| Accession | P02787 |
| Alternative Names | Serotransferrin; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 75.2 kDa |
| Expression Region | Val20-Pro698 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | Sterile water |
| Size | 10μg/100μg/1mg |
| Lead Time | Products are in stock,but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
- ✔ Low Endotoxin: Endotoxin level is ≤0.5 EU/mg, making it suitable for sensitive cell culture and biomanufacturing applications.
- ✔ Iron-Saturated Form: Provided as holo-transferrin, enabling efficient iron delivery and enhanced support for cell growth and metabolism.
- ✔ Optimized for Serum-Free Culture: Ideal for use in chemically defined and serum-free media, ensuring consistent and controlled experimental conditions.
Applications
Cell Culture
Widely used in serum-free and chemically defined media to support cell growth, viability, and proliferation.
Bioprocessing
Suitable for large-scale cell culture and biopharmaceutical production requiring consistent iron supplementation.
Cell Biology
Used to study iron metabolism, transferrin receptor function, and iron-dependent cellular processes.
Data Figures
Figure 1: Recombinant Human Transferrin (Cat. No. Y04501) SDS-PAGE under reducing (R) & Non-reducing condition. The gel was stained with Y04501 SDS-PAGE.
Figure 2: Recombinant Human Transferrin (Cat. No. Y04501) stimulates proliferation of MDCK cells in serum-free cell culture medium. The ED50 for this effect is 0.5-2 μg/mL. The bioactivity of Y04501 was higher than other competing products.
Figure 3: Highly consistent bioactivity between different production batches of Recombinant Human Transferrin (Cat. No. Y04501).
Figure 4: SEC-HPLC
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
