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$98.00
Sku: Y06201-10ug
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 08, 2026
Description
Recombinant Human Granulocyte Colony-Stimulating Factor (G-CSF) is a key hematopoietic cytokine that regulates the proliferation, differentiation, and activation of neutrophilic granulocyte precursors. It functions by binding to the G-CSF receptor (CSF3R), activating downstream signaling pathways such as JAK/STAT, PI3K/AKT, and MAPK, thereby promoting neutrophil production and survival. G-CSF plays a critical role in immune response and hematopoietic recovery.
This recombinant protein is expressed in CHO cells and corresponds to the mature human G-CSF sequence (Thr31–Pro204), ensuring proper folding and biological activity. With high purity (≥95%) and strong bioactivity (ED50 ＜0.5 ng/mL; ≥1 × 10⁷ units/mg in NFS-60 cell proliferation assay), this product is suitable for research in hematology, immunology, and cell biology. The protein is supplied in a tag-free, lyophilized form for convenient storage and reconstitution.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y06201 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | The bioactivity is determined by the dose-dependent proliferation of murine NFS-60 cells is ＜ 0.5 ng/mL, corresponding to a specific bioactivity of ≥ 1 x 107 units/mg. |
| Accession | P09919 |
| Alternative Names | Granulocyte Colony-Stimulating Factor; G-CSF; Pluripoietin; Filgrastim; Lenograstim; CSF3; C17orf33; GCSF |
| Species | Human |
| Expression System | CHO |
| Mol. Weight | 18.7 kDa |
| Expression Region | Thr31-Pro204 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | PBS, 5% Mannitol and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of ≥95%, ensuring reliable and reproducible experimental results.
- ✔ High Biological Activity: Exhibits potent activity with ED50 ＜0.5 ng/mL and ≥1 × 10⁷ units/mg, validated in NFS-60 cell proliferation assay.
- ✔ Mammalian Expression System: Expressed in CHO cells to ensure proper folding and native-like biological functionality.
- ✔ Convenient and Reliable: Supplied in a tag-free, lyophilized form for easy reconstitution, stable storage, and compatibility with diverse experimental applications.
Applications
Hematology
Promotes proliferation and differentiation of neutrophil precursors, widely used in hematopoietic studies.
Immunology
Enhances innate immune responses through regulation of neutrophil production and function.
Cell Biology
Used to study cytokine signaling pathways such as JAK/STAT and mechanisms of cell proliferation and survival.
Data Figures
Figure 1: The activity is determined by the dose-dependent proliferation of murine NFS-60 cells is ＜0.5 ng/mL, corresponding to a specific activity of ≥ 1 x 107 units/mg.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
