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Description
FGF-2 (Fibroblast Growth Factor-2 (Basic)), also known as bFGF, is one of the earliest recognized members of the FGF family. Secreted by endothelial cells, smooth muscle cells, and macrophages, FGF-2 acts on a variety of cell types. As a crucial regulator of cell growth and differentiation, FGF-2 possesses biological activities such as pro-angiogenic, proliferative, chemotactic, and migratory effects, playing a vital role in processes of cell differentiation and organismal development.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y00351H |
| Purity | ≥95%, by SDS-PAGE (under reducing (R)&Non-reducing conditions,visualized by Coomassie staining). |
| Endotoxin | ≤20 EU/mg by the LAL method. |
| Bioactivity | Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast Cells. The ED50 for this effect ≤2.0 ng/mL. |
| Accession | P09038-4 |
| Species | Human |
| Expression System | E. coli |
| Mol. Weight | 16.5 kDa |
| Expression Region | Pro143-Ser288 |
| Tag Info | Tag free |
| Form | Lyophilized |
| Buffer | Lyophilized from a 0.2 μm filtered solution containing PB, 5% Trehalose and 0.01% Tween 80, pH7.4. |
| Size | 10μg/50μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at -20°C to -80°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Hematology
Supports angiogenesis in hematopoietic niches, indirectly influencing hematopoiesis.
Immunology
Modulates immune cell proliferation and tissue repair indirectly.
Stem Cell Research
Widely used to maintain pluripotency and promote proliferation of ESCs and iPSCs.
Cell Biology
Key regulator of proliferation, survival, migration, and differentiation.
Data Figures
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

