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Description
Recombinant Human Epidermal Growth Factor (EGF) is a crucial protein involved in the regulation of cell growth, proliferation, and differentiation. EGF binds to its receptor EGFR, initiating a cascade of downstream signaling pathways that influence cellular processes, making it an indispensable tool in research areas such as cell biology, wound healing, and oncology.
Features
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High Purity: This product is subjected to rigorous purification processes, achieving a purity level of over 95%, ensuring the reliability and reproducibility of experimental results.
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High Biological Activity: Multiple activity assays confirm that this product effectively stimulates the expected biological responses in target cells, suitable for a wide range of research applications.
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Batch Consistency: Manufactured under strict production and quality control protocols to ensure high consistency between batches, reducing variability in experimental outcomes.
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Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements and broadening its application scope in research.
Applications
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Cell Biology: Used to study cellular processes such as proliferation, differentiation, and survival, EGF is crucial in understanding fundamental cell biology.
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Wound Healing: EGF promotes the regeneration of epidermal tissues, making it a valuable component in research focused on wound healing and tissue repair.
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Oncology: Investigates the role of EGF in cancer cell proliferation and survival, providing insights into potential therapeutic targets for cancer treatment.
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Regenerative Medicine: Utilized in research aiming to develop therapies for tissue regeneration and repair, contributing to advances in regenerative medicine.
Specifications
| Cytokines | |
| Expression Host | HEK293 cells |
| Molecular Mass | Predicts a molecular mass of 7.0 kD. |
| QC Testing Purity | > 90 % as determined by SDS-PAGE. |
| Endotoxin | < 0.1EU per 1 μg of the protein by the LAL method. |
| Activity | Measured in a cell proliferation assay using Balb/c 3T3 cells. The ED₅₀ for this effect is ≤3 ng/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 6 % mannitol are added as protectants before lyophilization. |
| Stability | 24 months at 2℃ to 8℃ in lyophilized state. 6 months at -20℃ under sterile conditions after reconstitution. 12 months at -80℃ under sterile conditions after reconstitution. |
| Storage | Recommend to aliquot the protein into smaller quantities after reconstituting with water for injection, normal saline or PBS, and keep the diluted concentration above 100 μg/mL. Avoid repeated freeze-thaw cycles. |
Data Figures
Storage and Stability
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Packaging: Supplied in sterile lyophilized form for ease of storage and transport.
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Storage: Store at -20°C or below to maintain product stability and activity. Avoid repeated freeze-thaw cycles to prevent protein degradation.
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Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.
Instructions for Use
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Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
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Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
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Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
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This product is intended for research use only and is not suitable for clinical diagnostics or therapeutic applications.
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Handle the product according to provided instructions to preserve its activity and stability.
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Contact our technical support team if you encounter any issues or have questions regarding the product's use.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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