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$188.00
Sku: TL-613-0010
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 18, 2026
Description
The recombinant human Epidermal Growth Factor (EGF) protein is a small molecular weight (~7 kDa) protein produced via transient expression in HEK-293 cells, featuring a C-terminal polyhistidine tag and containing 53 amino acid residues stabilized by three disulfide bonds.
As the founding member of the EGF family, it exerts its potent mitogenic effects by binding with high affinity to the Epidermal Growth Factor Receptor (EGFR) on the cell surface. This binding stimulates EGFR's intrinsic tyrosine kinase activity, initiating downstream signaling cascades (e.g., increased intracellular calcium, enhanced glycolysis and protein synthesis, upregulation of specific genes) that drive the proliferation, differentiation, and survival of diverse epidermal, epithelial cells, and fibroblasts.
In the context of cell and gene therapy, EGF holds significant potential due to its strong tissue repair and regenerative properties. Key applications include promoting wound healing (e.g., oral, gastroesophageal ulcers), maintaining mucosal barrier integrity, and stimulating the growth of epidermal/epithelial tissues in tissue engineering and in vitro culture systems.
Potential oncogenic risk associated with EGF requires careful consideration.
Specifications
| Parameter | RUO Grade | GMP Grade |
|---|---|---|
| Catalog Number | TL-613-0010 | GMP-TL613-0050 / GMP-TL613-0100 |
| Synonyms | Epidermal growth factor, Urogastrone, URG, HOMG4 | Epidermal growth factor, Urogastrone, URG, HOMG4 |
| Accession | UniProt P01133-1 | UniProt P01133-1 |
| Expressed Region | Asn971–Arg1023 | Asn971–Arg1023 |
| Tag | C-terminal 6×His-tag | C-terminal 6×His-tag |
| Expression System | HEK293 cells | HEK293 cells |
| Molecular Weight | 7.04 kDa | 7.04 kDa |
| Purity | >90% (SDS-PAGE) | >90% (SDS-PAGE) |
| Endotoxin | <0.1 EU/µg | <0.1 EU/µg |
| Formulation | Lyophilized from PBS (pH 7.4), 6% mannitol | Lyophilized from PBS (pH 7.4), 6% mannitol |
Features
- ✔ High Purity: This product is subjected to rigorous purification processes, achieving a purity level of over 95%, ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Multiple activity assays confirm that this product effectively stimulates the expected biological responses in target cells, suitable for a wide range of research applications.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure high consistency between batches, reducing variability in experimental outcomes.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements and broadening its application scope in research.
Applications
Cell Biology
Used to study cellular processes such as proliferation, differentiation, and survival, EGF is crucial in understanding fundamental cell biology.
Wound Healing
EGF promotes the regeneration of epidermal tissues, making it a valuable component in research focused on wound healing and tissue repair.
Oncology
Investigates the role of EGF in cancer cell proliferation and survival, providing insights into potential therapeutic targets for cancer treatment.
Regenerative Medicine
Utilized in research aiming to develop therapies for tissue regeneration and repair, contributing to advances in regenerative medicine.
Data Figures
Storage & Stability
- Packaging: Supplied in sterile lyophilized form for ease of storage and transport.
- Storage: Store at -20°C or below to maintain product stability and activity. Avoid repeated freeze-thaw cycles to prevent protein degradation.
- Stability: Stable for long-term storage under recommended conditions, ensuring reliable performance in research applications.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Support: Contact technical support if assistance is required.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
