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$470.00
Sku: TL-607-0050
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 18, 2026
Description
BMP-4 is a disulfide-stabilized homodimeric growth factor and core member of the Bone Morphogenetic Protein (BMP) subfamily within the TGF-β superfamily. It specifically binds type I (BMPR1A) and type II (BMPR2) receptors to activate SMAD1/5/8-SMAD4 signaling, precisely governing mesodermal lineage commitment, neurogenesis, and angiogenesis.
This protein induces osteogenic differentiation of mesenchymal stem cells via Runx2/Osterix transcriptional programs and coordinates with PTHRP to regulate mammary ductal morphogenesis and hair follicle suppression. Its activity is tightly modulated by extracellular antagonists (e.g., Noggin/Chordin), with dysregulation linked to pathologies like Fibrodysplasia Ossificans Progressiva (FOP).
For cell therapy applications, BMP-4 serves as a pivotal differentiation inducer with dual functions:
- Temporal gradient dosing in 3D hiPSC cultures efficiently generates mesodermal progenitors for cardiac regenerative therapies;
- Synergy with ROCK inhibitors to engineer vascular networks in organoids, enhancing in vivo integration and vascularization of osteochondral grafts.
Produced via mammalian cell transient transfection and chromatographic purification, this product retains native dimeric conformation and full bioactivity.
Specifications
| Specification | Acceptance Criteria | Results |
|---|---|---|
| Appearance | Freeze-dried preparation; white, loose solid | Compliance |
| Identity | The electrophoretic bands should be consistent with the molecular weight of the product | Compliance |
| Visible Particles | No visible particles | Compliance |
| Moisture | ≤ 4.0% | 2.0% |
| Purity | > 90.0% | Compliance |
| Endotoxin | < 0.1 EU/μg | < 0.1 EU/μg |
| Mycoplasma | Negative | Negative |
| Sterility | No growth | No growth |
| Biological Activity | Induction of alkaline phosphatase production in ATDC-5 cells; ED50 ≤ 100 ng/mL | ED50 = 12.04 ng/mL |
Features
-
✔
ative Dimeric BMP-4 Activity
Disulfide-stabilized homodimer preserves full BMPR-SMAD1/5/8 signaling fidelity.
-
✔
High Differentiation Potency
Strong osteo- and mesoderm-inducing activity validated by ATDC-5 ALP assay (ED₅₀ ≈ 12 ng/mL).
-
✔
Mammalian Expression, Tag-Free
HEK293 production ensures proper folding and bioactivity without tag-related interference.
-
✔
Excellent Batch Consistency
Tight QC on purity, endotoxin, sterility, and mycoplasma supports reproducible differentiation outcomes.
Applications
MSC Osteogenic Differentiation
Drives Runx2/Osterix programs for bone and osteochondral tissue engineering.
iPSC Mesoderm Induction
Enables controlled generation of mesodermal progenitors for cardiac and musculoskeletal models.
Organoid & 3D Culture Systems
Synergizes with ROCK inhibitors to promote vascularized organoid formation.
Regenerative Medicine Research
Supports studies in bone repair, developmental biology, and lineage specification.
Stability & Storage
- Lyophilized powder: Store at −20 °C in the original sealed container to maintain stability.
- Reconstitution: Reconstitute with sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4) prior to use.
- Handling: After reconstitution, aliquot the solution and avoid repeated freeze–thaw cycles.
Cautions
• Research Use Only: This product is not intended for diagnostic or therapeutic use.
• Handling: Follow recommended storage and handling instructions to maintain product stability.
• Safety: Use appropriate PPE and standard laboratory safety practices when handling this product.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
