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$84.00
Sku: Y04542-100mg
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by March 18, 2026
Description
Transferrin is an important glycoprotein composed of two domains, each containing an iron-binding site—one at the N-terminus and one at the C-terminus. Each site can bind one Fe³⁺ ion with the cooperative involvement of one HCO₃⁻ ion. Its primary role is to transport and regulate iron metabolism, delivering iron to cells in a safe manner that avoids the toxicity associated with free iron ions. Iron is an essential cofactor for haemoglobin synthesis, electron transport chain components (such as cytochromes), and DNA synthesis enzymes, making it particularly critical for highly proliferative cells like cancer cells and stem cells. As a key additive in serum-free culture media, transferrin can substitute for serum as an iron source, ensuring cell survival and growth.
Specifications
| Product Details | |
|---|---|
| Cat. No. | Y04542 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤10 EU/mg by the LAL method. |
| Bioactivity | Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is 0.5-2 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives. |
| Accession | Q29443 |
| Alternative Names | Beta-1 metal-binding globulin Siderophilin |
| Species | Bovine |
| Expression System | CHO |
| Mol. Weight | 75.8 kDa |
| Expression Region | Asp20-Pro704 |
| Tag Info | Tag free |
| Formulation | Lyophilized |
| Buffer | Sterile water |
| Size | 100mg/1g |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Reconstitution | It is recommended to redissolve in sterile deionized water. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability | 36 months at 2°C to 8°C in lyophilized state;6 months at -20°C to -80°C under sterile conditions after reconstitution;7-10 days at 2°C to 8°C under sterile conditions after reconstitution;Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
Stem Cell Research
Supplies iron in culture media, supporting stem cell proliferation.
Cell Biology
Iron transport, supports metabolism and proliferation in vitro.
Data Figures
Figure 1: Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
