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$15.00
Sku: C00404-10ug
Availability:
QUICK SHIP
In stock & estimated to ship in 1-2 days by June 06, 2026
Description
This Recombinant Anti-Human CD3 monoclonal antibody (mAb) is specifically engineered for robust in vitro T cell activation and expansion. By targeting the CD3 subunit on the T cell surface and mimicking TCR signaling, this antibody directly stimulates T cell proliferation without the need for antigen presentation. It is widely utilized as a gold-standard reagent for polyclonal T cell expansion in immunotherapy workflows, including CAR-T cell therapy research, PBMC culturing, and downstream functional T cell assays. When combined with recombinant cytokines such as IL-2, it ensures high-efficiency amplification of target T cell populations.
Specifications
| Product Details | |
|---|---|
| Cat. No. | C00404 |
| Purity | ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining). |
| Endotoxin | ≤1 EU/mg by the LAL method. |
| Bioactivity | Measure its binding ability with Jurkat cells using flow cytometry. The ED50 of this effect is less than 10 ng/mL. |
| Host Species | Mouse |
| Reactivity | Human |
| Expression System | CHO |
| Mol. Weight | 150 kDa |
| Tag Info | Tag free |
| Formulation | Lyophilized state |
| Buffer | 0.22 μm-filtered solution containing PBS, pH7.4. |
| Size | 500μg/1mg |
| Lead Time | Products are in stock, but custom products may require further consultation. |
| Shipping | The product is shipped with blue ice. |
| Storage & Stability |
As supplied (Liquid form): • 12 months at -20°C to -80°C under sterile conditions. • 2-4 weeks at 2°C to 8°C unopened. • Avoid repeated freeze-thaw cycles. Store in aliquots. Note: This product is compatible with lyophilization processes. If lyophilized by user: • 36 months at -20°C to -80°C in lyophilized state. • 6 months at -20°C to -80°C under sterile conditions after reconstitution. • 7-10 days at 2°C to 8°C under sterile conditions after reconstitution. |
Features
- ✔ High Purity: This product undergoes stringent purification processes, achieving a purity level of over 95%,ensuring the reliability and reproducibility of experimental results.
- ✔ High Biological Activity: Verified through multiple activity assays, this product effectively induces the expectedbiological responses in target cells.
- ✔ Batch Consistency: Manufactured under strict production and quality control protocols to ensure highconsistency between batches.
- ✔ Animal-Free: Produced without the use of animal-derived components, meeting animal-free requirements.
Applications
T Cell Activation & Proliferation
Used as a primary signal to induce in vitro T cell activation and cross-linking, driving robust T cell proliferation in functional immunological assays.
CAR-T & Cell Therapy Research
An essential component for large-scale T cell expansion and manufacturing protocols in CAR-T cell therapy and adoptive cell transfer (ACT) workflows.
TCR Signaling & Functional Assays
Serves as a stimulating agent to investigate T-cell receptor (TCR) signaling pathways, cytokine release profiles, and immune checkpoint regulations.
Stem Cell Differentiation
Applied in the downstream functional validation and maturation of T cells derived from human pluripotent stem cells (iPSCs/ESCs) or hematopoietic stem cells.
Data Figures
Figure 1: Recombinant Anti-Human CD3 mAb (Cat. No. C00404) SDS-PAGE under reducing (R) & Non-reducing conditions. The gel was stained with C00404 SDS-PAGE.
Figure 2: Recombinant Anti-Human CD3 mAb (Cat. No. C00404) binding with Jurkat. The ED50 for this effect is less than 10 ng/mL.
Instructions for Use
- Dissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.
- Prepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.
- Do not mix with other similar products to avoid cross-reactivity and potential interference.
Cautions
• Research Use Only: This product is not suitable for clinical diagnostics or therapeutic applications.
• Preservation: Handle according to instructions to preserve its activity and stability.
• Support: Contact our technical support team if you encounter any issues or questions.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
