Rat Noradrenaline (Norepinephrine) ELISA Kit

Description

Accurate measurement of noradrenaline (norepinephrine, NE) levels is important for understanding catecholamine regulation, sympathetic nervous system activity, and neuroendocrine responses in animal research models. Changes in norepinephrine concentration may reflect alterations in stress response pathways, autonomic regulation, cardiovascular function, and neurotransmitter metabolism. Reliable quantification of rat NE levels requires a sensitive and reproducible assay capable of analyzing complex biological samples.

The Rat Noradrenaline (Norepinephrine) ELISA Kit is designed for quantitative detection of noradrenaline levels in rat biological samples using a double antibody sandwich ELISA method. This research-use-only ELISA kit provides a convenient solution for researchers performing rat norepinephrine analysis in serum, plasma, cell culture supernatants, and tissue homogenates.

Noradrenaline, also known as norepinephrine, is a major catecholamine neurotransmitter involved in neuronal signaling, sympathetic nervous system activation, and physiological stress responses. Measuring NE concentration in rat experimental samples can support studies focused on neuroscience, stress biology, cardiovascular regulation, pharmacological evaluation, and neuroendocrine mechanisms.

The Rat Norepinephrine ELISA Kit utilizes pre-coated microplate wells, HRP-labeled detection antibodies, and TMB colorimetric detection technology. After antigen-antibody binding and enzymatic reaction, the optical density is measured at 450 nm, and sample concentrations are calculated according to the generated standard curve.

This Rat Noradrenaline ELISA Kit is intended for Research Use Only (RUO) and is not designed for clinical diagnosis, therapeutic applications, or human testing.

Specifications

Features

• Rat-Specific Noradrenaline Detection: Designed for quantitative measurement of rat norepinephrine (NE) levels in experimental biological samples.

• Double Antibody Sandwich ELISA Format: Uses antibody-based capture and HRP-labeled detection systems to provide reliable quantitative analysis of noradrenaline concentrations.

• Sensitive Catecholamine Measurement: Provides a detection sensitivity of less than 0.1 ng/mL for studying low-abundance neurotransmitter biomarkers.

• Multiple Biological Sample Compatibility: Suitable for rat serum, plasma, tissue homogenates, and cell culture supernatants.

• Standard Curve-Based Quantification: Includes calibrated NE standards for calculating sample concentrations through OD450 measurement.

• Colorimetric Microplate Detection: Compatible with standard microplate readers using 450 nm absorbance detection.

• Research Applications in Catecholamine Biology: Supports neuroscience, stress response, cardiovascular, and pharmacological research involving noradrenaline signaling.

• Research Use Only: For laboratory research applications only and not intended for diagnostic or therapeutic use.

Assay Principle

The Rat Noradrenaline (Norepinephrine) ELISA Kit is based on a double antibody sandwich ELISA principle. Microplate wells are pre-coated with antibodies specific to noradrenaline. When rat samples or standards are added, NE binds to the capture antibodies immobilized on the plate.

After incubation and washing, an HRP-conjugated detection antibody is added to form an antibody-antigen-antibody complex. Following additional washing steps, TMB substrate is introduced. HRP catalyzes the color reaction, and the intensity of the generated color is measured at 450 nm.

The optical density value is proportional to the concentration of noradrenaline present in the sample. Sample concentrations are determined by comparing measured OD values with the NE standard curve.

Applications

The Rat Noradrenaline ELISA Kit provides quantitative detection of norepinephrine for a variety of laboratory animal research applications involving catecholamine signaling, autonomic regulation, and physiological responses.

• Rat Neuroscience Research: Measurement of norepinephrine levels to investigate neurotransmitter regulation, neuronal signaling pathways, and neurochemical changes in rat models.

• Rat Stress Response Studies: Analysis of NE concentration changes associated with physiological stress, environmental stimulation, and stress-related biological responses.

• Sympathetic Nervous System Research: Evaluation of sympathetic activation and autonomic nervous system regulation through quantitative rat norepinephrine detection.

• Cardiovascular Research: Investigation of catecholamine-related mechanisms involved in cardiovascular regulation, adrenergic signaling, and vascular responses.

• Neuroendocrine Studies: Research on hormone-neurotransmitter interactions and biological pathways involving catecholamine regulation.

• Pharmacological Research: Assessment of drug-induced changes in noradrenaline metabolism and neurotransmitter-related pathways in rat models.

• Animal Model Studies: Quantitative monitoring of norepinephrine levels in rat experimental models for physiological and biochemical research.

• Biomarker Analysis: Supports laboratory investigation of NE as a catecholamine biomarker in biological samples.

Sample Types

The Rat Noradrenaline (Norepinephrine) ELISA Kit is compatible with commonly used rat biological samples for catecholamine research. Proper sample preparation is essential for obtaining accurate and reproducible norepinephrine measurements.

• Rat Serum: Serum samples collected using appropriate tubes and centrifuged to separate serum from blood cells before analysis.

• Rat Plasma: Plasma samples collected with EDTA, citrate, or heparin anticoagulants for norepinephrine quantification.

• Rat Tissue Homogenate: Tissue samples homogenized with physiological saline and centrifuged to obtain supernatant for NE detection.

• Cell Culture Supernatant: Cell-derived biological fluids processed by centrifugation to remove particles and suitable for ELISA analysis.

• Other Rat Biological Samples: Additional experimental samples may be evaluated according to specific research requirements and sample preparation procedures.

Assay Workflow

The Rat Norepinephrine ELISA Kit provides a straightforward microplate-based workflow for quantitative NE analysis.

1. Bring all reagents and samples to room temperature before starting the assay. Remove the required microplate strips from the sealed package and equilibrate for approximately 20 minutes.

2. Add 50 μL of NE standards into standard wells to generate the calibration curve.

3. Add 10 μL of rat sample followed by 40 μL of sample diluent into sample wells.

4. Add 100 μL of HRP-conjugated detection antibody into each well except blank wells, then incubate at 37°C for 60 minutes.

5. Wash the plate thoroughly to remove unbound components. Proper washing is important for reducing background signal and improving assay consistency.

6. Add 50 μL substrate solution A and 50 μL substrate solution B to each well, then incubate at 37°C for 15 minutes protected from light.

7. Add 50 μL stop solution to terminate the reaction and measure absorbance at 450 nm within 15 minutes.

8. Calculate rat norepinephrine concentration using the standard curve generated from the provided NE standards.

Performance Characteristics

FAQ