Rat Bone Marrow Macrophages (Frozen Cells)

Description

Rat Bone Marrow Macrophages from Cell Biologics are isolated from Sprague-Dawley rat tibias and femurs and grown in tissue culture plates with Cell Biologics’ Cell Culture Medium. Cells at passage 0 are cryo-preserved and each vial contains 3 x10⁶ cells per ml. Rat Bone Marrow Macrophages are characterized by immunofluorescence staining using antibody of CD11b or F4/80. These cells can be plated on multiwell culture plates for experiments under the cell culture conditions specified by Cell Biologics. Primary Bone Marrow Macrophages can not be cultured indefinitely and can not be passaged. 

Suggested Medium

M3368 Complete Macrophage Medium w/ Kit (500 ml)

Storage

Cryopreserved Cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

RA-6030

Cell-Culture-Protocol-CB

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

Publication

Circ Res. 2015 Nov 6; 117(11): e80–e89.