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Sku: FS-Q2001-S
Probe qPCR Master Mix (Universal)——StarLighter
Description
Probe-based qPCR is a demanding technique: non-specific amplification and poor reproducibility of results are common problems in the laboratory, especially when target templates have a high GC content or are present at low copy numbers.
StarLighter Probe qPCR Master Mix (Universal) is a universal probe qPCR master mix based on a hot-start engineered Taq DNA polymerase and an optimized buffer. It effectively reduces non-specific amplification and significantly improves the efficiency of probe-based real-time PCR. With its high sensitivity and broad quantitative range, it enables accurate quantitative and qualitative analysis of target genes as well as SNP detection.
For laboratories performing gene expression analysis or SNP genotyping, this universal probe qPCR master mix offers excellent reproducibility and high reliability, and is compatible with a variety of qPCR probes.
Specification
| Catalog No. | Size | Packaging |
|---|---|---|
| FS-Q2002 | 5 ml | 4 × 1.25 ml per box |
| FS-Q2001-S | 1 ml | 1 tube per box |
Features
- Strong amplification specificity — reliable data results.
- High GC template amplification — amplifies complex templates that are difficult to amplify with ordinary master mixes.
- High amplification efficiency and earlier Ct value — target is detected earlier in the real-time PCR run.
- High signal-to-noise ratio and high sensitivity — suitable for low copy detection.
- Hot-start engineered Taq DNA polymerase with optimized buffer — effectively reduces non-specific amplification.
- Broad quantitative range — accurate quantitative and qualitative analysis of target genes, including SNP detection.
- Excellent reproducibility and high reliability — compatible with a variety of qPCR probes.
Application
StarLighter Probe qPCR Master Mix (Universal) is a universal probe qPCR master mix suitable for:
- Gene expression analysis — accurate quantification of target genes.
- SNP and allele typing — SNP detection and genotyping assays.
- Validation of sequencing and microarray results — confirmatory real-time PCR.
FAQ
What is StarLighter Probe qPCR Master Mix (Universal)?
It is a universal probe qPCR master mix based on a hot-start engineered Taq DNA polymerase and an optimized buffer, designed for high-sensitivity probe-based qPCR detection with a broad quantitative range.
How does this master mix reduce non-specific amplification?
The master mix is based on a hot-start engineered Taq DNA polymerase with an optimized buffer, which effectively reduces non-specific amplification and significantly improves qPCR efficiency.
Which qPCR probes are compatible with this master mix?
This universal probe qPCR master mix is compatible with a variety of qPCR probes.
How does it perform with high GC templates?
It provides strong high GC template amplification. In a test with the ApoE3 gene (197 bp, 71% GC) at template amounts of 50, 5, 0.5 and 0.05 ng, the master mix amplified the template effectively, while a supplier K product failed (Ct > 45).
What is the amplification efficiency of this master mix?
In a test with the reference gene Actin (179 bp) using serial dilutions, the Ct value was reached approximately 1–1.5 cycles earlier, with better efficiency and R² compared with the reference.
What is the low copy sensitivity of this master mix?
Using a 5-fold serial dilution starting from 100 ng, the master mix amplified effectively down to 0.032 ng, with a good linear relationship of Ct values.
How stable is this master mix?
In stability tests, after 30 freeze-thaw cycles, 5 days at 37 °C, 28 days at room temperature and 43 days at 4 °C, no significant decrease in signal intensity or Ct value was observed (tested on a Thermo Q6 instrument).
What applications is this master mix suitable for?
It is suitable for gene expression analysis, SNP and allele typing, and validation of sequencing and microarray results.
What are the available sizes?
The master mix is available as catalog No. FS-Q2002 (5 ml, packaged as 4 × 1.25 ml per box) and FS-Q2001-S (1 ml, packaged as 1 tube per box).
Can this master mix be used for SNP detection?
Yes. With its broad quantitative range and high sensitivity, it supports accurate quantitative and qualitative analysis of target genes as well as SNP detection.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
