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Sku: FS-Q3010
Probe-Based Multiplex qPCR Master Mix (Universal)——StarLighter
Description
Multiplex probe-based qPCR detection places high demands on the reagent: when several target sequences with very different GC contents are amplified in a single reaction, and the templates are derived from complex sample types, the performance of a conventional master mix often drops — some targets show weaker signals or lower sensitivity, and the reliability of the whole assay can be compromised.
StarLighter Probe-Based Multiplex qPCR Master Mix (Universal) is a multiplex qPCR master mix developed specifically for multi-target detection. The polymerase has been improved through enzyme modification and directed evolution screening, combined with an optimized buffer reaction system, resulting in significantly enhanced inhibitor tolerance, broad sample compatibility and wide GC compatibility. This probe-based multiplex qPCR master mix significantly improves the sensitivity, specificity and low-copy detection of multiplex targets, while delivering high reproducibility, excellent amplification linearity and accurate quantification of target genes.
For laboratories running pathogen detection or disease detection panels, this universal multiplex qPCR master mix keeps multiple targets in one reaction performing consistently — across templates extracted from fecal samples, exfoliated cells, blood, serum/plasma, plasmids and pathogens.
Specification
| Catalog No. | Size | Packaging |
|---|---|---|
| FS-Q3011 | 5 ml | 4 × 1.25 ml per box |
| FS-Q3010 | 1 ml | 1 tube per pack |
Features
- Developed specifically for multiplex qPCR — the polymerase is improved through enzyme modification and directed evolution screening with an optimized buffer reaction system, delivering high sensitivity, high reproducibility and stable performance for multi-target detection.
- Wide GC compatibility — supports targets with a GC content ranging from 25% to 70%, allowing assays with different GC contents to run in the same reaction.
- Enhanced inhibitor tolerance and broad sample compatibility — suitable for templates extracted from fecal samples, exfoliated cells, blood, serum/plasma, plasmids and pathogens, across multiple scenarios and applications.
- Higher fluorescence signal — produces stronger fluorescence signals and improved amplification curve shapes in multiplex reactions.
- Excellent multiplex performance on low template amounts — in multiplex amplification of four targets with different GC contents from 0.1 ng of gDNA, this master mix shows higher sensitivity than master mixes from other manufacturers.
- High specificity and low-copy detection — significantly improves the sensitivity, specificity and low-copy detection rate of multiplex targets.
- Simple 2× premix format — just add primers, probes and template to run the reaction.
Application
StarLighter Probe-Based Multiplex qPCR Master Mix (Universal) is suitable for:
- Pathogen diagnosis — multiplex detection of pathogen-related targets in a single reaction.
- Disease detection — simultaneous detection of multiple disease-related targets for efficient screening.
FAQ
What is StarLighter Probe-Based Multiplex qPCR Master Mix (Universal)?
It is a probe-based multiplex qPCR master mix developed specifically for multi-target detection. The polymerase has been improved through enzyme modification and directed evolution screening, combined with an optimized buffer reaction system, significantly enhancing inhibitor tolerance, sample compatibility and GC compatibility for multiplex reactions.
How many targets can be detected in a single reaction?
In multiplex amplification of four targets with different GC contents using 0.1 ng of gDNA as the starting template, this master mix demonstrates strong fluorescence signals, good amplification curve linearity and high sensitivity.
What GC content range does this master mix support?
It supports targets with a GC content ranging from 25% to 70%, which makes it suitable for multiplex assays whose targets differ widely in GC content.
Which sample types is this multiplex master mix compatible with?
It is compatible with nucleic acid templates extracted from fecal samples, exfoliated cells, blood, serum/plasma, plasmids and pathogens, covering multiple sample scenarios.
How does this master mix handle inhibitors in multiplex reactions?
The enzyme modification and directed evolution screening improve the polymerase configuration, and the optimized buffer reaction system enhances inhibitor tolerance, helping multiplex assays perform consistently with templates from complex sample sources.
Why does multiplex performance remain strong across different targets?
This probe-based multiplex qPCR master mix is optimized for multi-target detection: it produces higher fluorescence signals and better amplification curve shapes, and significantly improves the sensitivity, specificity and low-copy detection of multiplex targets with high reproducibility and excellent amplification linearity.
Is this master mix suitable for single-target assays as well?
This master mix is designed for multiplex probe-based qPCR. For single-target probe assays, StarLighter also offers the Probe qPCR Master Mix (Universal) and the High Sensitivity Probe qPCR Master Mix (Universal) in the same product line.
What is the format of this 2× premix?
It is a 2× premix: just add primers, probes and template, then run the multiplex reaction.
What are the available sizes?
The master mix is available as catalog No. FS-Q3011 (5 ml, packaged as 4 × 1.25 ml per box) and FS-Q3010 (1 ml, packaged as 1 tube per pack).
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
