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Sku: P310055-10 PCS

Description

Precast-GLgel Hepes Native-PAGE 4–15% (10-Well) is a ready-to-use, non-denaturing gradient gel designed for analyzing proteins in their native, functionally active forms. Built with an innovative glass plate design, it significantly reduces non-specific protein adsorption and yields sharper, clearer native protein bands.

This 4–15% HEPES-based precast gel is delivered fully prepared with matching Native-PAGE running buffer, allowing users to load samples directly and eliminating the need for manual gel preparation. The 4–15% gradient range provides excellent separation performance for medium to high molecular weight native proteins, including large native protein complexes and membrane protein complexes, which require gentle electrophoresis conditions to preserve their structure.

Specification

Full name Precast-GLgel Hepes Native-PAGE 4–15% 10-Well
Package 10 pcs
Components 10 pcs gel, 10 pcs Native-PAGE running buffer
Gel size 8 × 7.4 × 0.15 cm
Plate size 9.8 × 8.4 × 0.41 cm
Wells 10 wells
Buffer system HEPES buffer system
Maximum sample loading volume 60 µL
Recommended sample loading volume 30 µL

 

Features

  • Glass plate construction effectively reduces non-specific protein adsorption, producing clearer and sharper native bands.

  • Automatic gel filling technology ensures stable quality and excellent reproducibility across batches.

  • 4–15% native gradient gel provides broad-range separation suitable for medium to high molecular weight native proteins.

  • Ideal for membrane proteins and membrane protein complexes, which require gentle, non-denaturing conditions to preserve native structure.

  • Suitable for large native protein complexes, supporting the analysis of oligomers and multi-subunit assemblies.

  • SDS-free gel matrix, designed specifically for non-denaturing electrophoresis (Native-PAGE) and compatible with downstream native protein detection.

  • Precast and ready to use, eliminating gel preparation steps and saving significant laboratory time.

  • Long shelf life and convenient storage at 2–8°C.

Applications

  • Native-PAGE analysis of proteins in non-denatured form

  • Separation of medium to high molecular weight native proteins

  • Electrophoresis of membrane proteins and membrane protein complexes

  • Analysis of native multiprotein complexes

  • Western blot (WB) after native separation

  • Experiments requiring a 4–15% native gradient gel

Storage

2~8°C, do not freeze

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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