Precast Argose Gel, 1% Agarose, with GelRed® Nucleic Acid Gel Stain, 10,000X, with TAE

Features

This product is for nucleic acid electrophoresis developed by Biofargo Inc., and has the following features and advantages:

1. Ready to use: Each kit contains agarose, nucleic acid stain, electrophoresis solution, loading buffer and other reagents, which will save the trouble to purchase all these separately.

2. Time saving: It eliminates the hassle of making gel by yourselves, thus saving you as much as one hour’s time to do experiments.

3. Easy to handle: Gelred in the gel is a stain produced by Biofargo, which is safe and reliable. During electrophoresis there is no need to stain or poststain the solution, thus making it easier to handle and ready to use. It is recommended to use a voltage of 80~120 volt, or adjust it to a suitable voltage according to your experiment.

4. Expense saving: The agarose gel can be reused after the DNA fragments obtained, which will not affect the subsequent DNA connection and other reactions. 
5. Compatible: The agarose gel uses the buffer of TAE type, which is the same as that used in the laboratory！

Kit Components

1. Each package contains 10 blocks of gels. According to the number and volume of the wells, four gel concentrations are for selection as shown in the following table.

For other concentrations(0.75~2.5%), please consult with the suppliers or call (804)-529-2296. If you need the gels that are uncommon in size, we can design personally for your special needs based on the common size.

2. 400µl ×1 Optimized 6×DNA Loading Buffer, Cat. No. 10052

Optimized 6×DNA loading buffer is designed specifically for this gel. Combined with other reagents, it can make the map more clear and sharp.

Optimized 6×DNA loading buffer is used to process DNA samples before electrophoresis. Mix Optimized 6×DNA loading buffer with the DNA samples at a volume ratio of 1:5 completely before loading. With the buffer compositions optimized, the incorporated tracking dyes bomophenol blue and xylene cyanol FF help to visualize the DNA migration during electrophoresis. Glycerin ensures that samples gather at the bottom of the loaded wells; EDTA binds divalent metal ions and inhibits metal-ion-dependent nucleases. In agarose gel of 1.0%, the mobility rate of bromophenol blue is approximately the same as that of double-stranded DNA fragment of 300bp. and that of xylene blue FF is approximately the same as that of double-stranded DNA fragment of 4000bp.

3.Two pouches of TAE Instant Granules, Cat.No. 1002

TAE Instant Granule is white. Each pouch can be diluted to 1 L of 1× TAE buffer，which is easy to operate and use. TAE buffer is widely used in nucleic acid electrophoresis. It consists of TRIS acetate and EDTA. DNA molecules are negatively charged in the buffer above the isoelectric point and migrate toward the positive pole. TAE buffer is often used in electrophoresis to separate genomic DNA, macromolecule superhelix DNA and amplified DNA fragments. DNA fragments larger than 13kb can be separated effectively in TAE buffer.

4. 80µl×1 Optimized Marker, Cat.No. OM50 / OM100 / OM1000 / OM2000 / OM5000 / OM15000 / OM MarkerⅠ/ OM Marker Ⅲ

Optimized Marker is designed specifically for this gel. Combined with other reagents, it can make the map more clear and sharp. Each set of the product is matched with one specific Marker. Eight Markers ( OM50 / OM100 / OM1000 / OM2000 / OM5000 / OM15000 / OM MarkerⅠ/OM Marker Ⅲ) can be selected. These eight are ready to use and consist of 1×loading Buffer. If gel is 6 or 13 wells, the recommended Marker loading amount is 2.0~3.0µl；If gel is 8 or 18 wells, the recommended Marker loading amount is 1.0~2.0µl；If gel is 11, 25 or 50 wells, the recommended Marker loading amount is 0.5~1.0µl. Or load the other volume for the experiment for electrophoresis.

OM50 DNA Marker is composed of 8 linear double-stranded DNA fragments of 50bp, 100bp, 150bp, 200bp, 250bp, 300bp, 400bp and 500bp. 250bp is the brightening band, 5µl In the product, the content of each band is about 40ng, and the brightening band is about 100ng.

OM100 DNA Marker is composed of 12 linear double-stranded DNA fragments of 100bp, 200bp, 300bp, 400bp, 500bp, 600bp, 700bp, 800bp, 900bp, 1000bp, 1200bp and 1500bp. 500bp is the brightening band, 5µl In the product, the content of each band is about 50ng, and the brightening band is about 120ng. 

OM1000 DNA Marker is composed of 13 linear double-stranded DNA fragments of 250bp, 500bp, 750bp, 1000bp, 1500bp, 2000bp, 2500bp, 3000bp, 4000bp, 5000bp, 6000bp, 8000bp and 12000bp. 1500bp and 4000bp are the brightening bands, 5µl In the product, the content of each band is about 30ng, and the brightening band is about 80ng. 

OM2000 DNA Marker is composed of 6 linear double-stranded DNA fragments of 100bp, 250bp, 500bp, 750bp, 1000bp and 2000bp. 750bp is the brightening band, 5µl In the product, the content of each band is about 50ng, and the brightening band is about 120ng. 

OM5000 DNA Marker is composed of 8 linear double-stranded DNA fragments of 100bp, 250bp, 500bp, 750bp, 1000bp, 2000bp, 3000bp and 5000bp. 750bp is the brightening band, 5µl In the product, the content of each band is about 50ng, and the brightening band is about 120ng. 

OM15000 DNA Marker is composed of 8 linear double-stranded DNA fragments of 500bp, 1000bp, 2000bp, 3000bp, 4000bp, 6000bp, 8000bp and 15000bp. 4000bp is the brightening bands, 5µl In the product, the content of each band is about 30ng, and the brightening band is about 80ng. 

OM Marker Ⅰ DNA Marker is composed of 6 linear double-stranded DNA fragments of 100bp, 200bp, 300bp, 400bp, 500bp and 600bp. 400bp is the brightening band, 5µl In the product, the content of each band is about 40ng, and the brightening band is about 100ng. 

OM Marker Ⅲ DNA Marker is composed of 7 linear double-stranded DNA fragments of 200bp, 500bp, 800bp, 1200bp, 2000bp, 3000bp and 4500bp. 1200bp is the brightening band, 5µl In the product, the content of each band is about 40ng, and the brightening band is about 100ng.

Shipping and Storage

Ship and store the kit at 2~8℃. It will remain stable for six months. 

Ship Optimized 6×DNA loading buffer at 2~8℃，for short-time storage and at -20℃ for long-time storage. It will remain stable for one year. 

Ship TAE Instant Granule at 2~8℃ or room temperature and store at room temperature or -20℃. It will remain stable for two years. 

Ship Optimized Marker at 2~8℃ and store it at -20°C for long-time storage. It will remain stable for one year.

Procedure

1.Add one pouch of TAE Instant Granule into the cleaned beaker, dissolved completely with 600 ml distilled water under a magnetic stirrer. Pour the solution into 1L flask. Add distilled water to the solution until the total volume is 1L. The final solution is 1×TAE buffer. 

2.Take out one kit, take off the plastic package, reverse it, support the two edges with index and middle fingers of both hands, immerse it in the buffer with the opening downward and gently press the central part of the kit with two thumbs. Thus the gel will fall into the buffer with the side of the well upward. Move the gel to make the well end close to negative electrode of the electrophoresis cell. If bubbles are produced in the sample wells, try to remove them. 

3.x Optimized 6×DNA loading buffer and DNA sample at a volume ratio of 1:5. Carefully load prepared Marker and the mixed sample into the wells with pipette successively. 

4.Connect the electrophoresis cell to the power source according to the conventions: Red-Anode and Black-Cathode. Turn on the power source. Note that the DNA sample moves from the negative to the positive (the end near the wells that DNA samples are loaded in is negative). 

5.Determine whether to stop electrophoresis according to the migration of the tracking dyes.

6.Switch off the power source when the electrophoresis finishes. Visualize the band by using a gel documentation system and compare the size of the amplified product with that of Marker.

Attention

The sensitivity of macromolecular Gelred is 8 times that of EB, reducing the sample loading and making the bands sharper ! If it's just for testing, the recommended loading in prestained gel is 50~100ng DNA or 1~2 µl PCR product. If the DNA concentration is unknown, run 1/5 to 1/3 the amount you would load on an EB gel for the test. If you need to load more DNA, using low voltage, and extending the sol time when cutting and recycling the gel to ensure complete dissolution of the gel, only then can the recovery rate be guaranteed!!

Documents

Prestained Agarose Gel Electrophoresis Kit.pdf