PluriCryo - Protein-Free hESC/iPSC Cryopreservation Solution

Description

Maintaining long-term viability and pluripotent characteristics after cryopreservation remains a critical challenge in human pluripotent stem cell workflows. During freezing and recovery processes, iPSC and ESC cultures may experience reduced survival, cellular stress, delayed recovery, or changes in colony morphology, which can affect downstream stem cell research applications.

Biofargo PluriCryo is a protein-free cryopreservation medium specifically developed for human induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). The formulation provides cryoprotective components and pluripotent stem cell-supporting nutrients designed to reduce freezing and thawing stress while supporting recovery after cryopreservation.

Unlike conventional freezing solutions containing serum or animal-derived components, Biofargo PluriCryo provides an animal component-free and protein-free environment for pluripotent stem cell preservation. This defined formulation helps researchers establish more consistent hPSC cryopreservation workflows for stem cell banking, iPSC storage, and routine laboratory maintenance.

Biofargo PluriCryo supports both single-cell cryopreservation and clump-based cryopreservation approaches, providing flexibility for different human pluripotent stem cell culture workflows.

Specifications

Features

• Dedicated iPSC and ESC Cryopreservation Medium — Developed specifically for human pluripotent stem cell freezing workflows, including induced pluripotent stem cells and embryonic stem cells.

• Protein-Free and Animal Component-Free Formulation — Designed without protein components or animal-derived ingredients to reduce variability associated with conventional serum-containing cryopreservation systems.

• 10% DMSO Cryoprotection System — Contains 10% (v/v) DMSO as a cryoprotective component commonly used for stem cell freezing applications.

• Supports Single-Cell and Clump Cryopreservation — Compatible with both single-cell freezing and aggregate-based freezing approaches for flexible hPSC workflows.

• Low Endotoxin Cell Culture Grade — Manufactured with low endotoxin control and filtration through <0.2 μm processing.

• Supports Post-Thaw Recovery — Designed to help maintain recovery performance after thawing and support restoration of normal pluripotent stem cell colony morphology.

Performance Data

iPSC Single-Cell Cryopreservation Performance

Biofargo PluriCryo was evaluated using human iPSC single-cell cryopreservation conditions. After 12 months of cryogenic storage, cell viability remained above 80%. Following thawing with 10 nM Y-27632 supplementation, cells recovered normal colony morphology after one passage.

ESC Clump Cryopreservation Performance

Human ESC H9 cells were evaluated using clump-based cryopreservation. After 12 months of storage, viability remained above 80%. Following thawing, cells recovered normal colony morphology after routine recovery culture.

Long-Term Storage and Recovery Workflow

Biofargo PluriCryo supports long-term preservation workflows by enabling controlled freezing, storage, and recovery procedures commonly used for human pluripotent stem cell banking.

Applications

Biofargo PluriCryo is designed for researchers performing human pluripotent stem cell cryopreservation workflows that require consistent freezing, storage, and recovery conditions. The protein-free formulation supports iPSC and ESC preservation applications where maintaining post-thaw recovery performance and culture characteristics is important.

• Human induced pluripotent stem cell (iPSC) cryopreservation — Supports routine freezing and long-term storage of human iPSC cultures.

• Human embryonic stem cell (ESC) preservation — Suitable for ESC freezing workflows including aggregate-based cryopreservation.

• Human pluripotent stem cell banking — Provides a defined freezing environment for establishing and maintaining iPSC and ESC cell stocks.

• Single-cell cryopreservation workflows — Compatible with single-cell dissociation and freezing procedures commonly used in human pluripotent stem cell culture.

• Clump-based stem cell cryopreservation — Supports aggregate or clump freezing methods for researchers preferring reduced mechanical stress during cell handling.

• Long-term iPSC storage — Designed for cryogenic preservation workflows requiring recovery after extended storage periods.

• Stem cell research and characterization studies — Supports preservation of pluripotent stem cell resources used in laboratory research.

• Regenerative medicine research workflows — Provides a research-use cryopreservation solution for pluripotent stem cell development programs.

• Human pluripotent stem cell culture workflow optimization — Helps researchers standardize freezing and thawing procedures across iPSC and ESC experiments.

• iPSC and ESC recovery studies — Suitable for evaluating post-thaw viability and colony morphology recovery after cryopreservation.

Why Choose Biofargo PluriCryo?

Successful human pluripotent stem cell preservation requires more than simply reducing freezing damage. iPSC and ESC cultures are sensitive to cryopreservation conditions, and inconsistent freezing or recovery procedures may influence cell viability, recovery speed, and downstream experimental reliability.

Biofargo PluriCryo provides a protein-free cryopreservation medium specifically designed for pluripotent stem cell workflows. By combining a 10% DMSO cryoprotective system with an animal component-free formulation, the medium helps researchers establish more controlled iPSC and ESC freezing procedures.

With support for both single-cell and clump-based cryopreservation approaches, Biofargo PluriCryo provides flexibility for different human pluripotent stem cell culture strategies, from routine laboratory storage to stem cell banking workflows.

FAQ