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Disodium piperazine-1,4-diethanesulphonate
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Synonym(s): 1,4-Piperazinediethanesulfonic acid disodium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) disodium salt, PIPES disodium salt
Description
PIPES-2Na (Disodium Piperazine-1,4-Diethanesulfonate), biochemical grade, is a white crystalline powder with purity ≥ 98%. With a molecular weight of 346.3 and high water solubility, it dissolves to yield clear, colorless solutions suitable for sensitive biochemical applications. As the sodium salt form of PIPES (piperazine-N,N′-bis(2-ethanesulfonic acid)), PIPES-2Na serves as a reliable “Good’s buffer” that helps maintain stable pH under physiological conditions while minimizing interference with metal ions or biomolecules. Its very low UV absorbance makes it ideal for spectrophotometric assays, and its chemical stability supports reproducible results in a wide range of laboratory workflows.
Specifications
| Item | Value |
| Chemical Name | Disodium Piperazine-1,4-Diethanesulphonate |
| Appearance | White Crystalline Powder |
| CAS No. | 76896-02-7 |
| Molecular Formula | C8H16N2O6S2Na2 |
| Molecular Weight | 346.3 |
| Grade | Biochemical Grade |
| Purity | ≥98% |
| Moisture | ≤3.0% |
| Infrared Spectrum | Qualified |
| pH (1% Di H2O, 25℃) | 9.2–10.0 |
| 20% Water Solubility | Colorless, Clear, Transparent |
| A280 (0.1M in Water) | ≤0.050 |
| A260 (0.1M in Water) | ≤0.050 |
Features
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High purity & biochemical grade — ensures minimal impurities for consistent experimental results.
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Excellent solubility and clarity — dissolves in water to produce clear, colorless solutions.
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Stable pH near physiological range — supports cell culture, enzyme assays, and protein work.
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Minimal interaction with metal ions — suitable for metal-sensitive biological systems.
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Low UV/visible absorbance — compatible with spectrophotometric and fluorescence-based assays.
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Chemically stable — maintains integrity under typical laboratory conditions.
Applications
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Cell culture media and buffers — maintains stable pH for cell growth and maintenance.
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Protein purification and enzyme assays — provides reliable buffering for sensitive reactions.
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Electrophoresis and chromatography — effective as running buffer or buffer component.
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Microscopy and tissue fixation — preserves cellular structure without interfering with metals.
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Protein crystallization and structural biology — supports stable conditions for long-term experiments.
Safety Disclaimer
This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.
Documents
MSDS
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.

