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Specification
| Package | 5 KU/100 U/200 U/500 U |
| Full name | Pfu DNA Polymerase |
| Applications | Conventional PCR, high-fidelity PCR, site-directed mutagenesis, clonal expression. |
| Summary | This product is a high-fidelity high-temperature resistant DNA polymerase isolated from the pyrophilus furiosis, with a molecular weight of 90 kDa, with 3'→5' exonuclease activity, no 5'→3' exonuclease activity, and its amplification product is blunt-end without protruding A. The elongation velocity of DNA polymerization was 600 bp/min, and the PCR product was blunt-end. Other high-temperature DNA polymerases, such as Vent, DeepVent, Tli, UlTma, etc., have correction functions, but Pfu has a very low error rate in amplifying DNA among all DNA polymerases that have been discovered so far. |
| Features | The fidelity effect is 8 times higher than that of Taq enzyme; It is highly thermostable, and the viability of incubation at 95°C for 1 h still maintains more than 90%. Produces blunt-ended PCR products that bind modified nucleotides (e.g., biotin, digoxigenin, fluorescently labeled nucleotides). |
| Composition | Pfu DNA polymerase (5U/ul), 10X PCR Buffer（20mM MgSO4）. |
| Reaction Buffer | 10X PCR Buffer |
| Unit Definition | The amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble precipitates at 74°C for 30 min was defined as 1 activity unit (U) . |
| Concentration | 5 U/μl |
| Storage Buffer | 20 mMTris-HCl(PH8.2); 0.1mMEDTA; 0.1%Tween20; 0.1%NP-40; 1mMDTT; 100mM KCl; 50% glycerol. |
| Component | Pfu DNA polymerase (5U/ul), 10X PCR Buffer（20mM MgSO4）. |
Storage
-20°C
Documents
https://cdn.shopifycdn.net/s/files/1/0521/5312/2997/files/P310139_EN_P.pdf
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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