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Specification
| Full name | PDHA1 rabbit polyclonal antibody |
| Alternative names | 50 μl/100 μl |
| Reactivity | rabbit polyclonal |
| Applications | WB, IHC |
| Host | Rabbit |
| Clone type | rabbit polyclonal |
| Target Background | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Swissprot No | P08559 |
| Gene Accession | BC002406 |
| WB Predicted band size | Immunohistochemical analysis of paraffin-embedded Human tonsil tissue or PDHA1 antigen-treated (Neutralization experiment) using P112885(PDHA1 Antibody) at dilution 1/50 |
| WB Positive control | P112885-WB-1-P |
| WB Recommended dilution | 43 kDa |
| IHC predicted cell location | Predicted cell location: Cytoplasm |
| IHC positive control | Positive control: Human cervical cancer |
| IHC Recommed dilution | Recommended dilution: 50-300 |
Storage
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
WB PIC Description
P112885-WB-1-P
IHC PIC Description
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue or PDHA1 antigen-treated (Neutralization experiment) using P112885(PDHA1 Antibody) at dilution 1/50
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