Pandy’s Reagent for Cerebrospinal Fluid (CSF) Protein Screening

Description

Pandy’s Reagent is a classical phenol-based turbidity reagent used for the qualitative and semi-quantitative assessment of total protein and globulin levels in cerebrospinal fluid (CSF). The assay is based on protein precipitation induced by phenol, producing visible turbidity proportional to elevated protein concentrations.

This reagent has been widely referenced in clinical chemistry literature and is commonly applied in laboratory research, training, and internal quality control workflows involving CSF protein analysis. Pandy’s Reagent enables rapid visual screening and is often used as an initial test prior to more specific protein differentiation methods.

Specifications

Features

• Rapid visual screening of elevated CSF protein levels

• Phenol-based precipitation chemistry with clear turbidity grading

• Simple manual workflow, no instrumentation required

• Semi-quantitative interpretation based on turbidity intensity

• Compatible with confirmatory globulin differentiation assays

• Suitable for training, method demonstration, and internal QC

• Stable formulation for routine laboratory handling

Applications

• Cerebrospinal fluid (CSF) protein screening

• Detection of pathological protein elevation in CSF samples

• Educational and laboratory training demonstrations

• Internal quality control and method validation exercises

• Pre-screening prior to selective globulin assays (e.g., Nonne–Apelt reaction)

Principle of the Assay

Pandy’s Reagent contains phenol dissolved in water. Upon contact with CSF, phenol induces precipitation of proteins—primarily globulins, but also albumin and other protein fractions. The resulting degree of turbidity correlates with protein concentration and is visually classified into graded categories ranging from negative to strongly positive reactions.

Interpretation of Results

Specificity & Limitations

Pandy’s reaction is not globulin-specific and may also respond to albumin and other protein fractions present at elevated concentrations. For enhanced specificity toward globulins, it is recommended to perform an additional confirmatory assay, such as the Nonne–Apelt reaction.

Reagent Composition

• Phenol (800 mmol/L)

• Non-reactive stabilizing components

• Purified water (Aqua p.a.)

Storage & Handling

• Store at +10 to +25 °C

• Protect from contamination after opening

• Close container tightly after use

• Observe standard laboratory safety procedures when handling phenol-containing reagents

Quality Control

For internal quality control, operator training, and visual reference calibration, the use of control cerebrospinal fluid samples is recommended to ensure consistency in result interpretation.

Regulatory Notice

This product is not intended for human diagnostic use.
For laboratory research, training, and quality control purposes only.