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$129.00
Sku: 12972ES08
Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by September 10, 2026
Description
OnePot Pro DNA Library Prep Kit V4 is is an advanced enzymatic DNA library preparation kit designed for compatibility with both Illumina™ and MGI™ next-generation sequencing(NGS) platforms. By utilizing high-efficiency enzymatic fragmentation, this kit eliminates the need for traditional mechanical shearing methods such as sonication, streamlining the workflow and minimizing hands-on time.
The kit integrates DNA fragmentation and end-repair into a single-step reaction, significantly reducing overall library preparation time and cost. It is suitable for a broad range of sample types, including plant genomes, animal genomes, microbial genomes, and more, with a flexible DNA input range from 1 ng to 1 μg.
The optimized enzymatic fragmentation ensures consistent and uniform library fragment sizes across different species, minimizing variation and enhancing sequencing performance. The prepared libraries are fully compatible with Illumina™ or MGI™ adapters and primers, enabling seamless downstream sequencing on respective platforms.
Features
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Broad Sample Compatibility: Supports a wide range of input DNA from 100 pg to 1 µg, covering all common sample types.
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High Fragment Recovery: Post-fragmentation recovery rate exceeds 60%.
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Excellent Library Uniformity: Consistent library distribution across different species under the same preparation conditions.
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Strong Inhibitor Tolerance: High success rate of direct library preparation from complex samples containing inhibitors.
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Automation-Friendly: Single-use reagents remain stable for 4 weeks at 4°C or 25°C, facilitating automation.
Application
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DNA Library Preparation;
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Whole Genome Sequencing(WGS);
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Whole Exome or other Targeted Capture Sequencing;
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Amplicon Sequencing;
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Immunoprecipitation Sequencing;
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Metagenomic Sequencing;
Components
| Components No. | Name | 12972ES08 | 12972ES24 | 12972ES96 |
| 12972-A | Smearase™ Buffer 4.0 | 80 μL | 240 μL | 960 μL |
| 12972-B | Smearase™ Enzyme 4.0 | 80 μL | 240 μL | 960 μL |
| 12972-C | Ligation Enhancer 4.0 | 240 μL | 720 μL | 3 × 960 μL |
| 12972-D | Rapid DNA Ligase 4.0 | 80 μL | 240 μL | 2 × 480 μL |
| 12972-E | 2× Ultima HF Amplification Mix | 200 μL | 600 μL | 3 × 800 μL |
Storage
This product should be stored at -25~-15℃ for one year.
Workflow
Figure 1. The workflow of OnePot Pro DNA Library Prep Kit
Flexible and High-Efficiency Fragmentation
Figure 2. Fragment size distribution of 250 ng human gDNA using the 12972 fragmentation module.
Excellent Buffer Tolerance
Figure 3. Fragmentation performance of 12972ES in different reaction buffers(H2O (pH 7.0), 1×TE (pH 7.5–8.0), 1×AE (pH 8.3), and 1×EB (pH 8.5)).
Fragmentation reactions were performed using 12972ES with 500 ng of 293 gDNA at 30°C for 20 minutes in four different buffers. When 10 μL of each buffer was used with DEPC-treated water, the fragment sizes were similar to those in pure DEPC water. Using AE or EB as the full reaction buffer resulted in slightly larger fragments.
Broad Species Compatibility
Figure 4. Library uniformity testing across different species using 12972 under identical fragmentation conditions.
Different DNA samples were fragmented with 12972 under identical conditions. The libraries showed consistent sizes (~450 bp / ~320 bp) with good uniformity across species.
Excellent Sequencing Data Performance
Figure 5. Performance of 10X WGS Data on Human and Bovine Samples
12972 demonstrates higher gene coverage compared to Supplier W*. Both false chimeric rate and hairpin artifacts are lower or comparable to those of Supplier W*.
Strong Stability
Figure 6. Library yield stability after storage at 4°C and 25°C for 4 weeks.
No decrease in library yield was observed after 4 weeks of storage at 4°C and 25°C, highlighting the kit’s suitability for automation.
Documents
Safety Data Sheet
Manuals
FAQ
What is OnePot Pro DNA Library Prep Kit V4?
OnePot Pro DNA Library Prep Kit V4 is an enzymatic DNA library preparation kit designed for next-generation sequencing (NGS) workflows. It is compatible with both Illumina™ and MGI™ sequencing platforms. The kit integrates DNA fragmentation and end repair into a single-step reaction, eliminating the need for mechanical DNA shearing methods such as sonication and simplifying the overall DNA library preparation workflow.
Which sequencing platforms is this DNA library prep kit compatible with?
The kit is designed for DNA library preparation on both Illumina™ and MGI™ next-generation sequencing platforms. Libraries generated using this kit are compatible with Illumina™ or MGI™ adapters and primers for downstream sequencing.
What DNA input range does OnePot Pro DNA Library Prep Kit V4 support?
According to the product manual, the kit supports DNA inputs ranging from 100 pg to 1 μg (1000 ng), making it suitable for a wide range of DNA sample amounts used in NGS library preparation.
What sample types are compatible with this enzymatic DNA library preparation kit?
The kit is suitable for a broad range of genomic DNA samples, including:
- Plant genomic DNA
- Animal genomic DNA
- Microbial genomic DNA
The product documentation states that the enzymatic fragmentation system provides consistent library fragment sizes across different species.
Does this DNA library prep kit require mechanical DNA fragmentation?
No. OnePot Pro DNA Library Prep Kit V4 uses high-efficiency enzymatic DNA fragmentation and does not require traditional mechanical shearing methods such as sonication.
What are the main advantages of enzymatic DNA fragmentation in this kit?
According to the product documentation, the enzymatic fragmentation system provides:
- Integrated DNA fragmentation and end repair in one reaction
- Reduced library preparation time and cost
- Consistent fragment size distribution
- Uniform library construction across different species
- High fragment recovery with recovery rates exceeding 60%
What sequencing applications is this DNA library preparation kit suitable for?
The kit is intended for multiple NGS library preparation applications, including:
- DNA Library Preparation
- Whole Genome Sequencing (WGS)
- Whole Exome Sequencing (WES)
- Targeted Capture Sequencing
- Amplicon Sequencing
- Immunoprecipitation Sequencing
- Metagenomic Sequencing
What is included in OnePot Pro DNA Library Prep Kit V4?
The kit contains the following core reagents:
- Smearase™ Buffer 4.0
- Smearase™ Enzyme 4.0
- Ligation Enhancer 4.0
- Rapid DNA Ligase 4.0
- 2× Ultima HF Amplification Mix
How does the workflow simplify DNA library preparation?
The kit combines DNA fragmentation and end repair into a single reaction before adapter ligation, followed by cleanup or size selection, library amplification (when required), and final quality control. This streamlined workflow reduces hands-on operation during DNA library preparation.
Can the DNA fragment size be adjusted?
Yes. The product manual provides recommended fragmentation times that generate different insert size ranges. Users can adjust the fragmentation time according to their desired library insert size requirements.
Does this DNA library prep kit provide consistent fragmentation across different species?
Yes. The product data demonstrate that genomic DNA from different species generates libraries with consistent fragment size distributions under identical fragmentation conditions, indicating good library uniformity across species.
Is the enzymatic fragmentation system compatible with different DNA buffers?
Yes. Product performance data show fragmentation performance in multiple reaction buffers, including H2O, TE, AE, and EB buffers. Similar fragment sizes were obtained when using H2O, TE, or AE under the tested conditions, while AE and EB used as the complete reaction buffer produced slightly larger fragments.
Does OnePot Pro DNA Library Prep Kit V4 support automation?
Yes. According to the product data, single-use reagents remained stable after storage for four weeks at both 4°C and 25°C without a decrease in library yield, supporting automated DNA library preparation workflows.
What library quality characteristics are reported for this DNA library preparation kit?
The product documentation reports the following performance characteristics:
- Fragment recovery rate exceeding 60%
- Uniform library distribution across different species
- Strong inhibitor tolerance for direct library preparation from complex samples
- High success rate with inhibitor-containing samples
How should the final DNA libraries be evaluated?
The manual recommends evaluating final libraries by measuring both library concentration and fragment size distribution. Library concentration can be measured using fluorescent methods such as Qubit or PicoGreen, while qPCR is recommended for more accurate quantification of sequence-ready libraries. Library size distribution can be analyzed using instruments such as the Agilent Bioanalyzer or equivalent capillary electrophoresis systems.
How should OnePot Pro DNA Library Prep Kit V4 be stored?
The unopened kit should be stored at -25°C to -15°C and has a storage life of one year under the recommended storage conditions.
Is this DNA library preparation kit intended for diagnostic use?
No. The product documentation and Safety Data Sheet state that OnePot Pro DNA Library Prep Kit V4 is intended For Research Use Only (RUO) and is not intended for diagnostic procedures.
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
