NP-40 Lysis Buffer

Description

NP-40 Lysis Buffer is a mild non-denaturing cell lysis buffer designed for efficient protein extraction while maintaining native protein structure and protein-protein interactions. During protein sample preparation, overly harsh extraction conditions may disrupt protein complexes or affect antibody-based enrichment workflows, while insufficient lysis can reduce protein recovery. This NP-40 cell lysis buffer provides a balanced extraction environment to help researchers prepare reliable cell and tissue lysates for Western blot, SDS-PAGE, immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and ELISA applications.

Formulated with a nonionic detergent system containing NP-40, this protein extraction buffer enables gentle disruption of cellular membranes while supporting non-denaturing protein extraction. Compared with stronger RIPA lysis buffers, NP-40 Lysis Buffer is particularly suitable for experiments requiring preservation of protein complexes, native protein interactions, and cytoplasmic protein analysis.

NP-40 Lysis Buffer is compatible with a wide range of biological samples, including animal cells, tissues, plant samples, bacteria, and yeast. It is part of a protein extraction reagent portfolio designed to provide researchers with flexible lysis solutions according to sample type, protein characteristics, and downstream experimental requirements.

Specifications

Features

• Mild non-denaturing protein extraction: Provides gentle cell lysis conditions to help maintain native protein structure and protein-protein interactions.

• NP-40 based cell lysis system: Uses a nonionic detergent formulation for efficient membrane disruption with reduced impact on protein complexes.

• Broad sample compatibility: Suitable for protein extraction from animal cells, tissues, plant samples, bacteria, and yeast.

• Optimized for antibody-based workflows: Compatible with immunoprecipitation (IP), co-immunoprecipitation (Co-IP), and protein interaction studies.

• Ready-to-use protein extraction reagent: Simplifies preparation of whole-cell lysates and tissue protein extracts for downstream analysis.

Applications

NP-40 Lysis Buffer is widely used as a mild protein extraction reagent in molecular biology, cell biology, and biochemical research workflows requiring efficient lysis with preservation of protein interactions.

• Western Blot Protein Analysis: Preparation of cell lysates and tissue extracts for SDS-PAGE separation and Western blot detection of target proteins.

• Immunoprecipitation (IP): Provides a suitable lysis environment for antibody-based protein enrichment while maintaining native protein conditions.

• Co-Immunoprecipitation (Co-IP): Supports protein-protein interaction studies by helping preserve protein complexes during extraction.

• Native Protein Extraction: Suitable for experiments requiring non-denaturing protein extraction and functional protein analysis.

• Cell Protein Extraction: Used for preparation of whole-cell lysates from adherent and suspension cultured cells.

• Tissue Protein Extraction: Supports extraction of proteins from animal and plant tissue samples after mechanical disruption.

• ELISA Sample Preparation: Provides protein lysates suitable for antibody-based detection workflows.

• Protein Interaction Research: Used in biochemical studies involving protein complexes, signaling pathways, and cellular regulation mechanisms.

• Bacterial and Yeast Protein Extraction: Can be combined with mechanical disruption or enzymatic treatment for microbial protein preparation.

Protocol Overview

For optimal protein extraction performance, sample processing should be performed under cold conditions to minimize protein degradation.

• Add appropriate protease inhibitors or phosphatase inhibitors before use according to experimental requirements.
• Lyse samples on ice or at 4 °C to maintain protein stability.
• Incubate cells or tissue samples with NP-40 Lysis Buffer until sufficient disruption is achieved.
• Centrifuge lysates at 10,000–14,000 ×g for 3–5 minutes and collect the clarified supernatant for downstream applications.

Because NP-40 Lysis Buffer contains detergent components, Bradford protein assays may produce inaccurate results. Detergent-compatible methods such as BCA protein assays are recommended for protein concentration measurement.

Ordering Information

FAQ