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Description
The Ni-NTA 6FF Pre-Packed Gravity Column (5mL) is a high-performance resin designed for the purification of His-tagged recombinant proteins. This resin is based on a 6% cross-linked agarose matrix, covalently coupled to Nitrilotriacetic acid (NTA), which forms a tridentate chelating agent that binds Ni²+ ions through three coordination sites. This ensures high-affinity binding and efficient purification of His-tagged proteins from various expression systems, including Escherichia coli, yeast, insect cells, and mammalian cells.
The column supports both native and denaturing conditions during purification and provides versatility in elution strategies, whether with a low pH buffer, imidazole, or even histidine solution. With an average bead size of 90 μm and a protein binding capacity of 10-40 mg of His-tagged protein per 1 mL resin, the Ni-NTA 6FF column ensures reliable and reproducible results with minimal non-specific binding.
This resin is compatible with all commonly used buffers, detergents, and denaturants, making it a reliable choice for researchers. The column’s stability across a wide pH range (2-14) makes it adaptable to various experimental conditions. Additionally, the resin can be regenerated and reused up to 5-10 times, making it a cost-effective solution for ongoing purification needs.
Specification
| Item | Value |
| Composition | 5 mL (1/5/10 columns) |
| Substrate | 6% Agarose |
| Average Particle Size | 0 |
| Particle Size | 45 μm - 165 μm |
| Protein Binding Ability | Approximately 10-40 mg Histidine Labeled Protein / 1 mL Resin |
| Maximum Flow Rate | 150 cm/h |
| Pressurization | 0.3 MPa, 3 bar |
| Storage Buffer | 20% Ethanol |
| Component | 5 mL (1/5/10 columns) |
| Storage | 4-8°C, do not freeze. Store in 20% ethanol solution |
Features
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High-Affinity Purification: The Ni-NTA resin binds Ni²+ ions via three coordination sites, offering a high-affinity interaction for His-tagged proteins.
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Broad Compatibility: Suitable for purification from multiple expression systems (E. coli, yeast, insect, and mammalian cells), under both native and denaturing conditions.
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Optimal Purity: The resin offers minimal non-specific adsorption, enabling high-purity isolation of His-tagged proteins.
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Wide pH Stability: Remains stable in pH 2-14, with short-term use for up to 2 hours at extreme pH values.
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Protein Binding Capacity: 10-40 mg of His-tagged protein per 1 mL resin, depending on conditions and protein characteristics.
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Regenerative and Reusable: The column can be reused 5-10 times, providing excellent cost efficiency.
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Chemical Compatibility: Stable in most commonly used buffers, detergents, and denaturants; however, avoid chelating agents like EDTA, EGTA, citrate, and histidine.
Applications
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His-tagged protein purification: Ideal for purifying recombinant proteins from E. coli, yeast, insect, and mammalian cells.
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Protein refolding: Suitable for refolding proteins from denatured states during purification.
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Affinitive chromatography: Uses metal affinity chromatography for high-purity protein isolation in biotech, pharmaceutical, and biochemistry labs.
Storage
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Storage Temperature: Store at 4-8°C; do not freeze.
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Storage Buffer: The resin is preserved in 20% ethanol to maintain activity.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
