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Specification
| Package | 1 KIT |
| Full name | Ni-NTA 6FF Sefinose(TM) Resin Kit |
| Applications | Purification of histidine labeled proteins from various expression sources such as Escherichia coli, yeast, insect cells, and mammalian cells. |
| Summary | The matrix of this medium has an average particle size of 90 μm 6% highly cross-linked agarose of m, with metal ion chelating ligands covalently linked, exhibits good stability. The chelating ligand carries divalent Ni2+, which is the medium for purifying most histidine labeled proteins. The ligand density of this medium has been optimized to ensure high protein binding capacity and high selectivity, while the high flow rate characteristics make it suitable for large-scale purification. |
| Features | Compared to Ni IDA agarose made from trivalent metal ion chelating agent iminodiacetic acid, the non-specific adsorption of Ni NTA agarose is significantly reduced, allowing users to obtain higher purity target proteins; 2. Support column refolding of denatured inclusion body proteins, achieving the effect of simultaneous separation and refolding; 3. Can tolerate a certain concentration of reducing reagents（ β- Mercaptoethanol can be used up to 20 mM; 4. The agarose matrix remains stable within the pH range of 2-14 and can withstand repeated in situ cleaning (CIP); 5. Minimal leakage of Ni2+during the purification process; 6. It can be reused and regenerated 5-10 times. |
| Composition | 1Kit (1 ml X 10 columns + 500 ml Elution Buffer + 500 ml Binding/Wash Buffer) |
| Substrate | Highly cross-linked 6% agarose |
| Average Particle Size | 90 μm（45-165 μm） |
| Particle Size | 0 |
| Protein Binding Ability | Approximately 10-40 mg histidine labeled protein/1 mL resin |
| Maximum Flow Rate | 150 cm/h |
| Pressurization | 0.3 MPa, 3 bar |
| Storage Buffer | 20% ethanol |
| Component | 1Kit (1 ml X 10 columns + 500 ml Elution Buffer + 500 ml Binding/Wash Buffer) |
Storage
2~8°C, do not freeze
Documents
https://cdn.shopifycdn.net/s/files/1/0521/5312/2997/files/P310131_P310132_P310129_P310127_EN_P.pdf
When can I expect my order to ship?
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We also provide express shippping for time-sensitive deliveries.
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