MycGuard™ MycGuard-2 Solution (500x), for Disinfecting Ordinary Water Bath

Notes: It is primarily composed of cationic peptide antibiotics, specifically designed to eliminate bacteria, fungi, mycoplasma, and other microorganisms in water baths.

Description

Mycoplasma contamination not only involves places that directly contact cells such as cell culture plates, culture reagents, or ultra-clean benches but also equipment such as water trays and water baths in cell culture incubators that may cause mycoplasma contamination. In addition to providing scavenging agents that directly remove mycoplasma agents, normal cells are also provided with mycoplasma preventive agents to prevent mycoplasma contamination. It mainly includes the following aspects: reagent prevention (directly added to the cell culture medium), ultra-clean bench prevention (spray), water bath prevention (directly added to the water bath), and cell incubator water tray prevention.

It is primarily composed of cationic peptide antibiotics, specifically designed to eliminate bacteria, fungi, mycoplasma, and other microorganisms in water baths. Its mechanism of action involves physically breaking down the cell walls of microorganisms and inhibiting the synthesis of enzymes required for their reproduction, ultimately leading to microbial death. As a result, this product does not induce antibiotic resistance. Multiple independent validation tests have shown that this product effectively clears various types of bacteria, fungi, mycoplasma, and other microorganisms. It is especially effective in inhibiting airborne bacteria, fungi, spores, and spore-forming bacteria for up to 7–10 days. It is provided as a 500× concentrated solution and can be added directly to the water bath at the recommended dilution.

Specifications

Key Features

• Formulated for open laboratory water baths. For the water pan of a CO₂ incubator, use MycGuard™-1 Solution (100×) instead.
• 500× concentrate: One 100 mL bottle treats up to 50 L of bath water.
• Non-corrosive and non-volatile — safe for your equipment: Compatible with the stainless steel tank, heating elements, and fittings of standard laboratory water baths, so continuous use will not pit, stain, or degrade the bath.
• Broad-spectrum control of bacteria, fungi, and mycoplasma.
• Cationic antimicrobial peptides physically disrupt the microbial cell wall rather than inhibiting a single metabolic target, so repeated use does not select for antibiotic-resistant strains.
• Coordinated treatment and degradation cycle: A single treatment supports contamination control for 7–10 days. Over the same period, the active peptides gradually degrade and antimicrobial activity tapers off, which is why re-dosing is recommended every 7–10 days or whenever the bath water is changed.
• Acute safety assessed in mouse and rabbit models with no adverse reactions observed.

Precautions

• For research use only. Not for human, veterinary, diagnostic, or therapeutic use.
• Do not add to cell culture media, buffers, or any solution that will contact cells.
• Do not use in the water pan of a CO₂ incubator. Use MycGuard™-1 Solution (100×) for that application — it is formulated for incubators and used at a different dilution.
• This is an environmental control product. It does not treat an already contaminated culture.
• Store at room temperature with the container tightly closed. Refrigeration and freezing are not required.
• Avoid contact with eyes and skin; rinse thoroughly with water if contact occurs.

Instructions for Use

1. Put on a lab coat and latex gloves.
2. Drain and clean the water bath following the equipment manufacturer’s instructions.
3. Refill with distilled or deionized water to the normal operating level.
4. Add MycGuard™-2 at a 1:500 dilution — 2 mL of concentrate per 1 L of water.
5. Stir gently to distribute, then resume normal operation.
6. Replace the treated water every 7–10 days, or per your incubator manufacturer's schedule if it is more frequent.

Storage

Store at room temperature

FAQ