MVM Analysis Kit SHENTEK®

For Research Use Only (RUO)

$1,010.00

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Sku: 1506742
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In stock & estimated to ship in 3-7 days by June 11, 2026
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Analysis Method
qPCR
Assay Time
Refer to manual
Storage Temperature
-20 °C (stable for 24 months)

Product Description

The SHENTEK® MVM Analysis Kit is a real-time PCR based qualitative assay serving as an analytical quality control reagent engineered exclusively for the molecular evaluation of MVM target sequences (covering strains i, p, m, and c) in industrial cell lines and related bioprocessing matrices (examples: CHO, BHK, NS0). Optimized for compatibility with the SHENTEK® Nucleic Acid Extraction Kit (Product No. 1506730), the assay includes an MVM-specific primer/probe mix, an internal process control (IPC), and a positive control to monitor nucleic acid recovery and amplification. Combined with SHENTEK extraction, the kit enables the efficient recovery and highly sensitive analysis of target DNA from complex matrices such as cell suspensions and in vitro cell culture supplements. The assay demonstrates high specificity, with no cross-amplification observed against tested cell lines and non-target genomes, achieving a limit of testing (LoD) of 50 copies per reaction. The kit is supplied for 50 reactions.

Technical Specifications

Parameter Details
Intended use Qualitative detection of Minute Virus of Mice (MVM) strains i, p, m and c in mouse-derived cell lines and serum
Kit size Reagents for 50 reactions
Analytical sensitivity (LOD) 50 copies per reaction
Specificity No cross-amplification observed with tested engineered cell lines (CHO, VERO, 293T, MDCK, NS0, Sf9) or engineered bacteria genomes (E. coli, Pichia pastoris)
Reaction volume 30 µL total reaction volume (20 µL qPCR MIX + 10 µL purified sample / control / NTC)
qPCR mix composition (per reaction) qPCR Master MIX 15.9 µL; MVM Primer & Probe MIX 2.4 µL; VIR IPC MIX 1.7 µL; (add 0.5 µL VIR IC to mix if not added during extraction); total qPCR MIX = 20 µL
Thermal cycling conditions Activation: 95 °C 10:00; 40 cycles of: Denaturation 95 °C 00:15, Annealing/Extension 60 °C 01:00 (fluorescence read during annealing/extension)
Controls provided / required VIR Internal Control (IC) included; Positive Control (MVM Positive Control) is referenced (Product No. 1506747) and recommended; Negative control (DDB or 1×PBS) and NTC required
Acceptance criteria — control wells NTC: FAM undetected; VIC: Ct < 35 for duplicate runs. NCS: FAM undetected; VIC: Ct < 35. PCS: FAM Ct < 35 and VIC Ct < 35
Acceptance criteria — test samples Positive: FAM Ct < 40 (at least one well) with specific amplification and VIC Ct < 35 in duplicates. Negative: FAM undetected in duplicates and Ctsample - CtNCS < 2. Not conclusive / possible inhibition: Ctsample - CtNCS ≥ 2 (compare VIC Ct of sample to NCS); retest or remove inhibitors
Compatible instruments (examples) SHENTEK-96S, LightCycler 480 II, ABI 7500 and most real-time PCR systems supporting probe assays
Storage & stability Kit components stored under recommended conditions (primarily -20 °C where indicated). Components stable up to 24 months (check label expiration date)

Features

  • Broad MVM strain coverage: Targets and detects four MVM strains (i, p, m and c).
  • High analytical sensitivity: Reported limit of detection of 50 copies per reaction.
  • High specificity: No cross-amplification with common mammalian cell lines or tested microbial genomes.
  • Internal process control included: VIR Internal Control (IC) monitors extraction and PCR inhibition.
  • Supplied controls: MVM Positive Control provided (reference product number supplied separately); NTC and NCS workflows specified.
  • Compatible with automated extraction: Recommended for use with rHCDpurify System and the SHENTEK® Virus DNA & RNA Extraction Kit to efficiently recover viral DNA from complex matrices.
  • Ready-to-use primer/probe mix: Pre-mixed MVM primer & probe simplifies setup and reduces assay variability.
  • Validated qPCR program: Predefined cycling conditions and instrument setup guidance provided for common platforms.

Applications

  • Molecular evaluation of industrial cell lines (e.g., CHO, BHK, NS0) utilized in biopharmaceutical production
  • Analysis of cell culture supernatant and in vitro cell culture supplements for MVM target sequences
  • In-process monitoring and lot-release analysis support within in vitro research environments
  • In vitro research studies requiring the qualitative molecular analysis of MVM target sequences

Kit Contents

VIR Internal Control (IC) (Part No. NNA057) — 600 µL × 1 tube-20 °C
DNA Dilution Buffer (DDB) (Part No. NND001) — 1.5 mL × 1 tubeRefer to label
qPCR Master MIX (Part No. NNB023) — 850 µL × 1 tube-20 °C, protect from light
MVM Primer & Probe MIX (Part No. NNC106) — 150 µL × 1 tube-20 °C
VIR IPC MIX (Part No. NNC107) — 100 µL × 1 tube-20 °C
* Total: 50 Reactions | Method: qPCR

Attention

• For Research Use Only — consult compliance statement.

• Read Material Safety Data Sheets (MSDS) and follow handling instructions.

• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).

• Handle positive control and extraction in a biological safety cabinet and follow BSL-2 laboratory safety guidelines.

• Use nuclease-free consumables and certified reagents.

• Thaw kit components at 2–8 °C or on ice; vortex and briefly centrifuge before use.

• Avoid cross-contamination: use separate areas and aerosol-resistant tips for reagent preparation, sample handling, and amplification.

• If VIC internal control Ct of sample is >2 cycles different from NCS VIC Ct, suspect PCR inhibition; retest or treat sample to remove inhibitors.

• Check expiration dates on component labels and store components under appropriate conditions (see labels).

Quality Management & Certifications

Quality System

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QMS (ISO, GMP)

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Quality Advantages

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Quality Control Process

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📂 Technical Resources & Downloads

PDF Product Manual ↓ PDF MSDS / SDS ↓

Frequently Asked Questions (FAQ)

Q1: What is the limit of detection (LOD) of the SHENTEK MVM Detection Kit?
The reported analytical sensitivity is 50 copies per reaction.
Q2: Which MVM strains does this kit detect?
The assay is designed to detect MVM strains i, p, m and c.
Q3: What sample types are supported and what extraction is recommended?
The kit is intended for cell culture and serum samples (example: 200 µL sample input). Use the SHENTEK® Virus DNA & RNA Extraction Kit (Product No. 1506730) and rHCDpurify System for nucleic acid extraction as recommended.
Q4: How are results interpreted and what indicates inhibition?
Positive: FAM Ct < 40 (at least one well) with specific amplification and VIC Ct < 35. Negative: FAM undetected in duplicates and Ctsample - CtNCS < 2. If Ctsample - CtNCS ≥ 2 (VIC comparison), this suggests PCR inhibition; retest or remove inhibitors.

Research Use Only

✔

Research Use Only (RUO) – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.

✔

Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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