MSCryo – Serum-Free Mesenchymal Stem Cell Cryopreservation Solution

Description

Maintaining reliable cell viability after cryopreservation is a critical challenge in stem cell research workflows. During freezing, long-term storage, transportation on dry ice, and thawing procedures, mesenchymal stem cells (MSCs) may experience osmotic stress, ice crystal damage, and reduced recovery performance, which can affect downstream expansion and differentiation studies.

Biofargo MSCryo Serum-Free MSC Cryopreservation Medium is specifically developed for mesenchymal stem cell freezing and long-term storage applications. The formulation provides cryoprotective support during low-temperature preservation while helping maintain post-thaw cell viability and recovery performance.

Unlike conventional serum-containing freezing solutions, Biofargo MSCryo is formulated without serum, animal-derived components, or protein ingredients, reducing the risk of undefined components and improving consistency for controlled MSC banking workflows.

Designed as a serum-free MSC freezing medium, Biofargo MSCryo supports cryopreservation workflows for mesenchymal stem cells, induced pluripotent stem cells (iPSCs), and embryonic stem cells (ESCs), providing researchers with a reliable solution for cell storage and recovery.

Specifications

Features

• Serum-free MSC cryopreservation medium designed for mesenchymal stem cell freezing workflows

• Animal component-free formulation reducing undefined biological components

• Protein-free composition supporting controlled cryopreservation conditions

• Contains 10% DMSO for effective cryoprotective performance

• Supports long-term MSC storage and stem cell banking applications

• Maintains high post-thaw cell viability after cryopreservation

• Suitable for MSC, iPSC, and ESC cryopreservation research

Performance Data

Post-Thaw Viability and Long-Term Cryopreservation Performance

Biofargo MSCryo was evaluated using human umbilical cord-derived mesenchymal stem cells (UC-MSCs) to assess the performance of serum-free MSC cryopreservation. Cells were cultured under serum-free stem cell culture conditions and cryopreserved after passage 4 (P4).

Following controlled freezing at -80°C and subsequent storage in liquid nitrogen, thawed cells demonstrated stable recovery performance. Biofargo MSCryo maintained post-thaw cell viability above 90% during evaluated storage periods, including 1-month, 3-month, and 12-month cryopreservation conditions.

Long-term storage evaluation showed that cells preserved with Biofargo MSCryo maintained high recovery performance, with more than 80% cell recovery after 12 months of cryopreservation.

MSC Cryopreservation Recovery Analysis

Successful MSC banking requires not only cell survival during freezing but also efficient recovery after thawing. Biofargo MSCryo supports recovery workflows by minimizing cryopreservation-related stress and maintaining the ability of thawed MSCs to resume culture expansion.

The performance data demonstrates that Biofargo MSCryo provides a reliable solution for researchers requiring consistent MSC freezing, storage, and post-thaw recovery.

Applications

Biofargo MSCryo Serum-Free MSC Cryopreservation Medium is designed for laboratories performing stem cell preservation workflows where consistent viability, controlled formulation, and reliable recovery are important considerations.

• Cryopreservation of human mesenchymal stem cells (MSC)

• Long-term storage of MSC research cell banks

• Human umbilical cord-derived MSC preservation

• Bone marrow-derived MSC cryopreservation research

• Adipose-derived MSC freezing workflows

• Stem cell banking and inventory management

• Post-thaw MSC expansion and recovery studies

• iPSC cryopreservation research applications

• Embryonic stem cell (ESC) preservation studies

• Regenerative medicine and cell therapy research workflows

Why Choose Biofargo MSCryo?

Maintaining a stable MSC population during long-term storage requires a cryopreservation medium that balances effective cryoprotection with controlled formulation characteristics. Variations in freezing conditions, storage duration, and thawing procedures may influence cell recovery and downstream experimental reproducibility.

Biofargo MSCryo provides a serum-free MSC freezing solution designed to support reliable cryopreservation workflows. The animal component-free and protein-free formulation helps reduce variability associated with undefined biological components, making it suitable for laboratories requiring consistent MSC storage conditions.

With validated performance in human mesenchymal stem cell cryopreservation, Biofargo MSCryo supports researchers working on MSC expansion, differentiation studies, cell banking, and stem cell research applications.

Recommended Cryopreservation Workflow

MSC Freezing Procedure

• Harvest cultured cells after completion of the expansion process and collect cells by centrifugation.

• Remove culture medium and resuspend cells with Biofargo MSCryo cryopreservation medium.

• Adjust final cell concentration to 10⁵–10⁷ cells/mL, with 10⁶–10⁷ cells/mL recommended for routine freezing.

• Transfer cell suspension into cryovials and perform controlled cooling using a freezing container.

• Store at -80°C overnight before transferring samples into liquid nitrogen (-196°C) for long-term storage.

MSC Thawing Procedure

• Rapidly thaw frozen cells using a 37°C water bath or automated cell thawing system.

• Transfer thawed cells into a centrifuge tube and add at least three volumes of culture medium.

• Centrifuge to remove cryopreservation medium and continue downstream MSC culture.

FAQ