Mouse Tail SuperDirect™ PCR Kit(room temperature lysis)

Descprition

This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tail and mouse ear samples for PCR reaction, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at room temperature (20-25°C). No heating is required, and other processes such as protein and RNA removal are not required. The released trace amounts of DNA can be used as Template for PCR reaction.

2×M-PCR EasyTM Mix has strong tolerance to PCR reaction inhibitors, and can use the lysate of the sample to be tested as a template for efficient and specific amplification. This reagent contains Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Features

• No time-consuming and expensive DNA purification is required.

• The sample demand is small, just take mouse tails (or mouse ears with a diameter of 2-5mm) above 2mm.

• Easy to operate, it can be lysed without heating to inactivate the protease, and the lysed product can be directly subjected to PCR reaction.

• Foregene Mouse Tail Genomic DNA Extraction Kit (DE-05211) can be used to extract genomic DNA from the processed rat tail.

• Optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

Storage

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

Reagent Foregene Protease Plus I has a unique formula. To ensure its better activity and stability, please store it at 4℃.

Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

Reagent 2×M-PCR EasyTM Mix, if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).