Mouse Noradrenaline (Norepinephrine) ELISA Kit

Description

Accurate quantification of noradrenaline (norepinephrine, NE) levels is essential for studying catecholamine signaling, sympathetic nervous system activity, and neuroendocrine regulation in mouse experimental models. Changes in norepinephrine concentration may reflect alterations in stress response pathways, autonomic function, cardiovascular regulation, and neurotransmitter metabolism. Reliable detection of mouse NE levels requires a sensitive and reproducible assay compatible with complex biological samples.

The Mouse Noradrenaline (Norepinephrine) ELISA Kit is designed for quantitative detection of noradrenaline levels in mouse biological samples using a double antibody sandwich ELISA method. This research-use-only ELISA kit provides researchers with a reliable solution for mouse norepinephrine analysis in serum, plasma, cell culture supernatants, and tissue homogenates.

Noradrenaline, also known as norepinephrine, is an important catecholamine neurotransmitter involved in neuronal signaling, sympathetic nervous system activation, and physiological stress responses. Measurement of NE concentration in mouse samples supports studies related to neuroscience, stress biology, cardiovascular regulation, pharmacological evaluation, and neuroendocrine mechanisms.

The Mouse Norepinephrine ELISA Kit utilizes pre-coated microplate wells, HRP-labeled detection antibodies, and TMB colorimetric detection technology. After antigen-antibody binding, washing, and enzymatic reaction, the optical density is measured at 450 nm. Mouse NE concentrations are calculated by comparing sample OD values with the generated standard curve.

This Mouse Noradrenaline ELISA Kit is intended for Research Use Only (RUO) and is not designed for clinical diagnosis, therapeutic applications, or human testing.

Specifications

Features

• Mouse-Specific Noradrenaline Detection: Designed for quantitative measurement of mouse norepinephrine (NE) levels in biological research samples.

• Double Antibody Sandwich ELISA Format: Uses capture antibody and HRP-conjugated detection antibody technology for quantitative analysis of noradrenaline concentrations.

• Sensitive Catecholamine Quantification: Provides a sensitivity of less than 0.1 ng/mL for detecting low-level NE biomarkers in mouse samples.

• Compatible with Multiple Mouse Samples: Supports mouse serum, plasma, tissue homogenates, and cell culture supernatants.

• Standard Curve-Based Analysis: Includes NE calibration standards for quantitative concentration calculation through OD450 measurement.

• Colorimetric Microplate Detection: Compatible with standard laboratory microplate readers capable of measuring absorbance at 450 nm.

• Applications in Catecholamine Research: Suitable for mouse neuroscience, stress response, cardiovascular, neuroendocrine, and pharmacological studies.

• Research Use Only: Designed exclusively for laboratory research and not intended for diagnostic or therapeutic use.

Assay Principle

The Mouse Noradrenaline (Norepinephrine) ELISA Kit is based on a double antibody sandwich ELISA principle. Microplate wells are pre-coated with antibodies specific to noradrenaline. When mouse samples or standards are added, NE binds to the capture antibodies immobilized on the microplate.

After incubation and washing, an HRP-labeled detection antibody is added to form an antibody-antigen-antibody complex. Following removal of unbound components, TMB substrate is added. HRP catalyzes the color reaction, and the intensity of the generated signal is measured at 450 nm.

The OD value obtained from the assay is positively correlated with the concentration of noradrenaline in mouse samples. The final NE concentration is calculated by comparing sample values with the provided standard curve.

Applications

The Mouse Noradrenaline ELISA Kit supports quantitative measurement of norepinephrine in mouse models for studies involving catecholamine signaling, autonomic regulation, and physiological responses.

• Mouse Neuroscience Research: Measurement of norepinephrine levels to investigate neurotransmitter regulation, neuronal signaling pathways, and neurochemical changes in mouse models.

• Mouse Stress Response Studies: Analysis of NE changes associated with physiological stress, environmental stimulation, and stress-related biological mechanisms.

• Sympathetic Nervous System Research: Evaluation of sympathetic activation and autonomic nervous system regulation through quantitative mouse norepinephrine detection.

• Cardiovascular Research: Investigation of catecholamine-related mechanisms involved in cardiovascular regulation, adrenergic signaling, and vascular responses.

• Neuroendocrine Research: Study of interactions between neurotransmitter signaling and endocrine regulation pathways.

• Pharmacological Studies: Evaluation of drug-induced changes in norepinephrine metabolism and related signaling pathways in mouse models.

• Animal Model Research: Quantitative monitoring of NE levels in mouse experimental models for biochemical and physiological studies.

• Catecholamine Biomarker Analysis: Supports investigation of norepinephrine as a biomarker in mouse biological samples.

Sample Types

The Mouse Noradrenaline (Norepinephrine) ELISA Kit is compatible with commonly used mouse biological samples for catecholamine research. Proper sample collection and preparation help improve the accuracy and reproducibility of mouse NE quantification.

• Mouse Serum: Serum samples collected using suitable tubes and centrifuged to separate serum from blood cells before analysis.

• Mouse Plasma: Plasma samples collected with EDTA, citrate, or heparin anticoagulants for norepinephrine detection.

• Mouse Tissue Homogenate: Tissue samples homogenized with physiological saline and centrifuged to obtain supernatant for NE measurement.

• Cell Culture Supernatant: Cell-derived biological fluids processed by centrifugation to remove particles and suitable for ELISA analysis.

• Other Mouse Biological Samples: Additional experimental samples may be evaluated according to specific research requirements and sample preparation procedures.

Assay Workflow

The Mouse Norepinephrine ELISA Kit provides a simple microplate-based workflow for quantitative detection of noradrenaline levels in mouse samples.

1. Allow all reagents and samples to reach room temperature before beginning the assay. Remove required microplate strips from the sealed package and equilibrate for approximately 20 minutes.

2. Add 50 μL of NE standards into standard wells to establish the calibration curve.

3. Add 10 μL of mouse sample followed by 40 μL of sample diluent into sample wells. The sample dilution factor should be considered during final concentration calculation.

4. Add 100 μL of HRP-conjugated detection antibody into each well except blank wells, then incubate at 37°C for 60 minutes.

5. Wash the microplate thoroughly to remove unbound reagents. Complete washing is important for reducing background signal and maintaining assay performance.

6. Add 50 μL substrate solution A and 50 μL substrate solution B into each well. Incubate at 37°C for 15 minutes protected from light.

7. Add 50 μL stop solution to terminate the reaction. Measure absorbance at 450 nm within 15 minutes using a microplate reader.

8. Calculate mouse norepinephrine concentration by comparing sample OD values with the standard curve generated from NE standards.

Performance Characteristics

FAQ