Mannitol Agar,1kg

Description

Beef extract and peptone acts as source of carbon, nitrogen, minerals, vitamins and other essential growth nutrients. Mannitol is the fermentable carbohydrate. Phenol red is a pH indicator and change the color from red to yellow if mannitol is fermented and acid products are produced.  Typical pathogenic staphylococci (coagulase-positive staphylococci) ferment mannitolandform yellow colonies with yellow zones around the colonies. Typical non-pathogenic staphylococci do not ferment mannitol andform red colonies. Sodium Chloride, in high concentration, inhibits most bacteria other than staphylococci.

Cultural characteristics after 18-48 hours at 35-37°C.

Components (for making 4.55L medium)

Part I:

Protease pepton  45.5g 
 Sodium Chloride  341.25g 
 D-Mannitol  45.5g 
 Beef Extract  4.55g 
 Phenol Red  0.115g

Part II: 

Agar  68.25g

Usage

Must dissolve all contents of Part I in distilled water. Adjust pH=7.4-7.5, make final to 4.55L.  Add Part II (Agar).

Sterilize by autoclaving at 121°C for 15 minutes. Pour plates.

Storage

Store dehydrated powder, in a dry place, in tightly-sealed containers at 2-25°C.