0 item(s)
View cart
You have no items in your shopping cart.
Description
The kit adopts magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality total RNA from various tissues, cells and blood. The product can be perfectly matched with Kingfisher Flex 96 and TGuide S32 automated nucleic acid extractors. Magnetic beads are adsorbed, transferred and released by special magnetic rods, thus realizing the transfer of magnetic beads and nucleic acids and improving the automation degree. The whole experimental process is safe and convenient, with high purity extracted total RNA, free from contamination of genomes, proteins and other impurities. If high-throughput automated extraction is required, TIANGEN Company can provide an integration plan.
Features
-
The kit can meet the requirements of manual extraction as well as batch extraction on various high-throughput platforms.
-
The products obtained by the kit meet the requirements of downstream detection experiments and NGS analysis.
Applications
RNA purified by the kit is suitable for RT-PCR, Real Time RT-PCR, chip analysis, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, molecular cloning and other downstream experiments.
Kit Contents
| Contents | 50 preps |
| Buffer RL | 30 mL |
| Buffer RD | 48 mL |
| Buffer RW | 2 × 12 mL |
| RNase-Free ddH2O | 15 mL |
| MagAttract Suspension W | 2 × 1 mL |
Required Reagents
β-mercaptoethanol, ethanol.
Storage
The kit can be stored for 15 months under dry conditions at room temperature (15-30°C) and stored at 2-8℃ for a longer time.
Important Notes
-
Before operation, add β-mercaptoethanol to Buffer RL to a final concentration of 1%, for example, add 10 μl of β-mercaptoethanol to 1 ml of Buffer RL. This buffer is best prepared right before use. The prepared Buffer RL can be stored at 2-8°C for 1 month. The Buffer RL may form precipitates during storage, and if any precipitates appear, please heat and dissolve before use.
-
Add 96-100% ethanol to Buffer RD and Buffer RW before the first use. Please refer to the label on the bottle for the added volume.
Experimental Example
Optional Reagents and Tools
DNase I (Cat.no. GRT411); Magnetic Stand (Cat.no. OSE-MF-01); 10×Red cell Lysis Buffer H; RNase-Free Columns CS set.
Self-provided Reagents and Instruments
β-mercaptoethanol, isopropanol, 96-100% ethanol, homogenization equipment (mortar, electric homogenizer, etc.), magnetic stand.
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
No data
