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Package | 5 ML(10%V/V)/10 ML(10%V/V)/25 ML(10%V/V) |
Full name | Mag-Beads Strep-Tag II Protein Purification |
Applications | It can be used for purification of Strep-Tag II labeled proteins in various expression systems, including rod-shaped viruses, mammalian cells, yeast, and bacteria, suitable for most protein properties and facilitating analysis of protein protein protein interactions. |
Summary | Strep-Tag II is a small tag with 8 amino acids (WSHPQFEK), which is only about 1.06 kDa due to its small size and does not affect the structure and function of the fused protein. These mild purification parameters can maintain the biological activity of the protein and yield over 99% purity through only one step of extraction. Among numerous affinity tags, although Strep-tag II appeared later than Flag, c-Myc, and His tags, it has been widely used due to its high affinity, high single-step purification purity (better than His tags), mild purification conditions, and fusion of both ends of the protein. |
Features | The purification conditions are mild, and the single step purification purity is superior to His label, without affecting the structure and function of the fused protein, with high affinity. The binding ability of Strep Tag II tags is much higher than that of streptavidin. Compared to fillers, it is more efficient and faster to separate and purify labeled proteins. |
Composition | 5 mL (10%V/V)/ 10 mL (10%V/V)/25 mL (10%V/V) |
Substrate | Magnetic agarose microspheres |
Average Particle Size | 0 |
Particle Size | 30 μm~150 μm |
Protein Binding Ability | ≈7 mg Strep-tag II protein/mL filler |
Storage Buffer | 1×PBS,contain 0.1%Tween-20 |
Magnetic Bead Ratio | Magnetic beads suspended in protective solution with a content of 10% (V/V) |
Component | 5 mL (10%V/V)/ 10 mL (10%V/V)/25 mL (10%V/V) |
2~8°C, do not freeze
https://cdn.shopifycdn.net/s/files/1/0521/5312/2997/files/P310136_EN_P.pdf
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