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Description
Laboratory teams evaluating a Lipoxidase enzyme need clearly defined information about enzyme identity, biological source, physical form, solubility, activity, purity, storage, and assay conditions. Catalog No. BF-1980010 is Lipoxidase from soybean, identified as linoleate:oxygen oxidoreductase with Enzyme Commission number EC 1.13.11.12.
This soybean Lipoxidase is supplied in freeze-dried form and is readily soluble in distilled water or dilute buffer. Its listed activity is approximately 10 U/mg protein, and its listed purity is approximately 60%. The product should be stored at −20°C (−4°F).
Lipoxidase catalyzes the oxidation of polyunsaturated fatty acids containing cis,cis-1,4-pentadiene systems to form hydroperoxides. The supplied product information also provides a defined spectrophotometric activity method using linoleic acid at pH 9.0 and 25°C, with the change in absorbance measured at 232.5 nm.
The complete unit definition and assay conditions allow laboratories to interpret the stated Lipoxidase activity according to the method supplied with the product.
Specification
| Product Name | Lipoxidase |
|---|---|
| Systematic Name | Linoleate:oxygen oxidoreductase |
| EC Number | EC 1.13.11.12 |
| Catalog Number | BF-1980010 |
| Source | Soybean |
| Form | Freeze-dried |
| Solubility | Readily soluble in distilled water or dilute buffer |
| Activity | Approximately 10 U/mg protein |
| Purity | Approximately 60% |
| Storage | −20°C (−4°F) |
| Listed Price | US$15.00/g |
Lipoxidase Unit Definition
One unit will cause an increase in A232.5 of 0.001 per minute at pH 9.0 and 25°C when linoleic acid is the substrate in a 3.0 mL volume with a 1 cm light path.
One A232.5 unit is equivalent to the oxidation of 0.12 μmol of linoleic acid.
| Substrate | Linoleic acid |
|---|---|
| Assay Wavelength | 232.5 nm |
| Assay pH | pH 9.0 |
| Assay Temperature | 25°C |
| Reaction Volume | 3.0 mL |
| Light Path | 1 cm |
Assay Reagents
- 0.1 M ammonium hydroxide-ammonium chloride buffer, pH 9.0
- 1 mM linoleic acid
- Lipoxidase diluted to 0.6 U/mL
Assay Procedure
- Set the spectrophotometer, equipped with a strip chart recorder and temperature control, to 232.5 nm and 25°C.
- Pipette 2.9 mL of ammonium hydroxide-ammonium chloride buffer into the quartz cuvettes.
- Add 0.1 mL of the enzyme solution to the test cuvette, mix, and record the rate of absorbance at 232.5 nm for 5 minutes.
- Calculate ΔE232.5 nm/min from the initial linear portion of the curve.
Activity Calculation
Activity (U/mg) = (ΔE232.5 nm/min × Total Vol. × Enz. Diln.) ÷ (27.4 × Enz. Vol. × mg Enz./mL)
Features
- Lipoxidase from soybean
- Linoleate:oxygen oxidoreductase
- Enzyme Commission number EC 1.13.11.12
- Catalog number BF-1980010
- Supplied in freeze-dried form
- Readily soluble in distilled water or dilute buffer
- Activity of approximately 10 U/mg protein
- Purity of approximately 60%
- Storage at −20°C (−4°F)
- Defined spectrophotometric assay at 232.5 nm
- Defined activity assay using linoleic acid at pH 9.0 and 25°C
Application
Lipoxidase catalyzes the oxidation of polyunsaturated fatty acids containing cis,cis-1,4-pentadiene systems to form hydroperoxides.
The supplied product description states that the degradation of the hydroperoxide by Lipoxidase appears to prevent the breakdown of amino acids and proteins associated with an odorous carbonyl compound that produces the beany flavor found in many legumes.
The supplied information states that Lipoxidase is widely distributed and is especially concentrated in legumes, with soybeans having the highest concentrations.
Frequently Asked Questions
What is Lipoxidase Catalog No. BF-1980010?
Catalog No. BF-1980010 is Lipoxidase from soybean. Its systematic name is linoleate:oxygen oxidoreductase, and its EC number is EC 1.13.11.12.
What is the source of this Lipoxidase?
The source of Lipoxidase Catalog No. BF-1980010 is soybean.
What is the EC number of Soybean Lipoxidase?
The Enzyme Commission number listed for this Soybean Lipoxidase is EC 1.13.11.12.
What is the systematic name of this Lipoxidase enzyme?
The systematic name stated in the supplied product information is linoleate:oxygen oxidoreductase.
What is the physical form of Lipoxidase BF-1980010?
Lipoxidase Catalog No. BF-1980010 is supplied in freeze-dried form.
Is Soybean Lipoxidase soluble in water?
The product is readily soluble in distilled water or dilute buffer.
What is the activity of this Soybean Lipoxidase?
The listed activity is approximately 10 U/mg protein.
Is 10 U/mg protein a minimum guaranteed activity?
No minimum value is stated in the supplied product information. The activity is listed as approximately 10 U/mg protein.
What is the purity of Lipoxidase BF-1980010?
The listed purity is approximately 60%.
Is 60% a minimum guaranteed purity?
No minimum value is stated in the supplied product information. The purity is listed as approximately 60%.
How should Soybean Lipoxidase be stored?
The stated storage condition is −20°C, equivalent to −4°F.
How is one Lipoxidase activity unit defined?
One unit will cause an increase in A232.5 of 0.001 per minute at pH 9.0 and 25°C when linoleic acid is the substrate in a 3.0 mL volume with a 1 cm light path.
What does one A232.5 Lipoxidase unit represent?
One A232.5 unit is equivalent to the oxidation of 0.12 μmol of linoleic acid.
What substrate is used in the Lipoxidase activity assay?
Linoleic acid is used as the substrate.
At what wavelength is Lipoxidase activity measured?
The activity method measures the change in absorbance at 232.5 nm.
What pH and temperature are used in the Lipoxidase assay?
The assay is performed at pH 9.0 and 25°C.
What reaction does Lipoxidase catalyze?
Lipoxidase catalyzes the oxidation of polyunsaturated fatty acids containing cis,cis-1,4-pentadiene systems to form hydroperoxides.
What price is listed for Lipoxidase BF-1980010?
The supplied product information lists a price of US$15.00 per gram.
