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Sku: BF-94668660
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Availability:
AVAILABLE
In stock & estimated to ship in 3-7 days by April 08, 2026

Description

Influenza Virus A H3N3 Probe Realtime RT-PCR Detection and Typing Kit is a one-step RT-PCR kit intended for research use in nucleic acid analysis under controlled laboratory conditions using probe-based real-time PCR. Purified RNA is reverse transcribed into cDNA and subsequently amplified using sequence-specific primers and probes. During amplification, fluorescence signals are generated through probe cleavage, allowing real-time monitoring of amplification under controlled laboratory conditions. The resulting signals may be used for comparative analysis of target nucleic acids.

Note: Supplied without internal control. Internal control version can be customized based on your assay requirements.
This product was previously listed under catalog number 15-15930-15903 and is now updated to BF-94668660 for improved product management.

Key Features

  • One-step RT-PCR workflow to reduce handling steps and minimize contamination risk.

  • Demonstrates high sensitivity under optimized laboratory conditions.

  • High specificity based on primer-probe design.

  • Includes a non-infectious plasmid-based positive control for assay verification.

  • Compatible with commonly used RNA purification methods.

  • Suitable for research applications including assay development and method evaluation.

  • Broad dynamic range under laboratory conditions.

  • For research use only. Not intended for diagnostic or therapeutic use.

Kit Components

Component Name Description Size Vial Lid Color
2× qPCR Master Mix Contains optimized buffer and Taq polymerase mix 500 μL 0.5 μL Vial Natural
Primer-Probe Mix Target-specific primer and probe set 165 μL 1.5 mL Vial Brown
Positive Control (1E7 copies/µL) Non-infectious plasmid-based control 50 mL 0.5 mL Vial Yellow
Nuclease-free Water For reaction setup and kit blank control (no-template control) 800 μL 1.5 mL Vial Blue
Manual 1 Set NA NA

 

Application Areas

  • Molecular biology research: qPCR-based detection and quantification of target nucleic acids.
  • Assay development: Suitable for method development, optimization, and analytical performance evaluation.
  • Industry applications (research use): Widely used in biotechnology, pharmaceutical, academic, and agricultural research laboratories.
  • For Research Use Only (RUO). Not for use in diagnostic procedures.

Storage & Shipping

  • Store at - 20 °C

  • The product is shipped with dry ice to maintain low-temperature conditions and ensure product integrity during transit.

FAQ

Q: Are Biofargo PCR and qPCR Kits compatible with my thermal cycler or real-time PCR instrument?

A: Yes. Biofargo PCR Kits and probe-based qPCR Kits are designed for broad compatibility with mainstream instruments.

PCR Kits can be used with standard thermal cyclers from leading brands.

Probe-based qPCR Kits are fully compatible with major real-time PCR platforms, including ABI, Bio-Rad, Roche, QuantStudio, Qiagen Rotor-Gene Q (5-plex), and Genesig Q16.

Our technical team is available to assist with instrument setup and run optimization to ensure the best results.

Documents

Influenza Virus A H3N3 Probe Realtime RT-PCR Detection and Typing Kit User Manual

MSDS

When can I expect my order to ship?

Most orders are filled and shipped within 2-3 business days from the time they are received.

Our standard shipping usually take 2-5 days.

We also provide express shippping for time-sensitive deliveries. 

Email contact@biofargo.com if you have any requirements.

 

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