Immortalized RFP-Expressing Human Primary Carotid Artery Endothelial Cells

Description

Immortalized RFP-Expressing Human Primary Endothelial Cells from Cell Biologics are isolated from human tissue and grown in gelatin pre-coated tissue culture flasks with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. Immortalized RFP-Expressing Human Primary Endothelial Cells are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. 550389, BD), CD31/PECAM-1 (Catalog No. FAB3567P, R&D), or VE-Cadherin (FITC-VE-cadherin, Catalog No. 560411, BD), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended. 

 Immortalized cell lines from Cell Biologics are derived from primary cells expressing RFP. The primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).

 Red Fluorescent Protein (RFP), a mutant form of green fluorescent protein, gives rise to very bright red fluorescence when exposed to yellow-green light. Cell Biologics generates various RFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. RFP-expressing cells are developed through transducing RFP-lentiviral particles.

Suggested Medium

H1168                Complete Human Endothelial Cell Medium w/ Kit – 500 ml