Immortalized RFP-Expressing Canine Primary Vein Smooth Muscle Cells

Description

Long-term fluorescence-based smooth muscle cell research can be limited when primary cultures have a restricted expansion window or when cells are difficult to distinguish during imaging and mixed-culture workflows. Immortalized RFP-Expressing Canine Primary Vein Smooth Muscle Cells provide a canine vascular smooth muscle cell model with extended culture capability and bright red fluorescence for direct cell visualization.

These immortalized canine smooth muscle cells are derived from primary vein tissue of beagle dogs. The cells are cultured in gelatin pre-coated tissue culture flasks using Complete Smooth Muscle Cell Medium and are supplied as cryopreserved cells at a concentration of 0.5 × 10⁶ cells per mL.

RFP-expressing cells are developed through transduction with RFP-lentiviral particles. Red fluorescent protein produces bright red fluorescence when exposed to yellow-green light, supporting fluorescence microscopy, cell visualization and cell-tracking workflows in culture.

Cell identity is characterized by immunofluorescence staining with an α-smooth muscle actin antibody. The cells are documented as negative for bacteria, yeast, fungi and mycoplasma. Under the specified cell culture conditions, they can be expanded for more than 10 passages at a recommended split ratio of 1:2.

This cryopreserved canine vascular smooth muscle cell model is intended for research use only and is not authorized for human use, in vitro diagnostic procedures or therapeutic applications.

Specifications

Key Features

• Immortalized canine primary vein smooth muscle cells derived from beagle dog vein tissue
• RFP-expressing cell model developed through RFP-lentiviral particle transduction
• Bright red fluorescence under appropriate yellow-green excitation
• Characterized by α-smooth muscle actin immunofluorescence staining
• Expandable for more than 10 passages under the specified culture conditions
• Recommended 1:2 split ratio for routine cell expansion
• Documented negative for bacteria, yeast, fungi and mycoplasma
• Supplied cryopreserved at 0.5 × 10⁶ cells per mL
• Compatible with M2268 Complete Smooth Muscle Cell Medium w/ Kit
• Designed for research workflows requiring a fluorescent canine vascular smooth muscle cell model

RFP-Expressing Cell Model

Red fluorescent protein provides a visible fluorescence signal that helps researchers distinguish the canine smooth muscle cells during fluorescence-based observation. The datasheet describes these RFP-expressing cells as being generated through transduction with RFP-lentiviral particles.

The resulting fluorescent smooth muscle cell model may be incorporated into experiments in which direct visualization, location or observation of the cultured cells is required. The extended lifespan of the immortalized cell model also supports experiments that require more culture expansion than a limited-passage primary cell preparation.

Applications

Immortalized RFP-Expressing Canine Primary Vein Smooth Muscle Cells may support research workflows involving:

• Canine vascular smooth muscle cell research
• Canine vein smooth muscle cell culture
• Fluorescence microscopy and red fluorescence imaging
• Live-cell visualization under compatible imaging conditions
• RFP-based cell tracking in culture
• Visualization of fluorescent smooth muscle cells in mixed-cell or co-culture workflows
• Observation of cell distribution during in vitro culture experiments
• α-Smooth muscle actin-positive cell model research
• Extended cell expansion for repeat laboratory experiments
• Fluorescence-based comparison of cultured canine smooth muscle cells

The product datasheet does not provide validation for a specific disease model, contractility assay, therapeutic application or clinical use. Researchers should confirm suitability for their individual experimental design.

Cell Expansion and Culture Considerations

• Use gelatin pre-coated tissue culture vessels for cell growth.
• Use M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 mL as the suggested medium.
• Follow the specified cell culture conditions associated with the product.
• Use a recommended split ratio of 1:2 during expansion.
• The cells can be expanded for more than 10 passages under the specified conditions.
• Avoid repeated freezing and thawing because repeated freeze-thaw cycles are not recommended.
• Use appropriate aseptic cell culture procedures and handle animal-derived cells with caution.

Quality Control

Shipping and Storage

• Cryopreserved cells are shipped frozen on dry ice by overnight delivery.
• Upon arrival, transfer the frozen vial immediately to a liquid nitrogen storage tank at approximately -180°C.
• Do not use a -20°C or -80°C freezer for storage of the cryopreserved cells after arrival.
• Repeated freezing and thawing of the cells are not recommended.
• Live-cell shipment may be available on request.
• Treat all animal-derived cells as potential pathogens because no testing procedure can completely guarantee the absence of infectious agents.

Recommended Medium

M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 mL is the suggested medium listed for these immortalized RFP-expressing canine vein smooth muscle cells.

Use of the recommended medium and appropriate coating solution helps maintain the culture conditions specified for product recovery, cell expansion and testing.

Documents

D-6086IM.RFP Product Datasheet

Frequently Asked Questions