Immortalized RFP-Expressing Canine Primary Small Intestinal Smooth Muscle Cells

Description

Researchers selecting a fluorescent canine intestinal smooth muscle cell model need to confirm the tissue source, cell identity, expansion capacity, culture requirements, and storage conditions before beginning an experiment. Immortalized RFP-Expressing Canine Primary Small Intestinal Smooth Muscle Cells provide a primary-derived, RFP-expressing canine smooth muscle cell model with documented tissue origin, characterization, microbial testing, and extended culture capability.

These immortalized canine small intestinal smooth muscle cells are isolated from the small intestinal tissue of beagle dogs. They are grown in gelatin pre-coated tissue culture flasks using Complete Growth Medium. Cells are harvested at passages 2–3, cryopreserved, and delivered frozen. Each vial is specified as containing 0.5 × 10⁶ cells per mL.

Cell identity is characterized by immunofluorescence staining with an α-smooth muscle actin antibody. The cells test negative for bacteria, yeast, fungi, and mycoplasma. Under the specified cell culture conditions, they can be expanded for more than 10 passages using a recommended split ratio of 1:2.

These primary-derived immortalized cells have an extended lifespan. According to the product documentation, primary cells in this product series are immortalized through overexpression of either the simian virus 40 large T-antigen or human telomerase reverse transcriptase. The documentation does not identify which of these two immortalization methods is used specifically for Catalog No. D-6088IM.RFP.

RFP-expressing cells are developed through transduction with RFP-lentiviral particles. Red fluorescent protein produces bright red fluorescence when exposed to yellow-green light, providing a fluorescently identifiable canine smooth muscle cell model for applicable in vitro research workflows.

Specifications

Specification note: The product datasheet specifies a concentration of 0.5 × 10⁶ cells per mL. It does not state the total cryopreservation volume of each vial.

Features

• Primary-derived immortalized canine smooth muscle cells isolated from beagle small intestinal tissue
• RFP expression introduced through transduction with RFP-lentiviral particles
• Harvested and cryopreserved at passages 2–3
• Supplied as a frozen vial at a documented concentration of 0.5 × 10⁶ cells/mL
• Characterized by α-smooth muscle actin immunofluorescence staining
• Tested negative for bacteria, yeast, fungi, and mycoplasma
• Expandable for more than 10 passages under the specified culture conditions
• Recommended for subculture at a split ratio of 1:2
• Cultured using gelatin pre-coated tissue culture flasks
• Shipped frozen on dry ice overnight and stored in liquid nitrogen upon arrival

Applications

Immortalized RFP-expressing canine small intestinal smooth muscle cells are intended for in vitro research requiring a canine intestinal smooth muscle cell model with fluorescent identification and documented extended expansion under the specified culture conditions.

• In vitro research requiring canine small intestinal smooth muscle cells
• Research workflows requiring RFP-expressing canine cells for fluorescence-based observation
• Studies requiring primary-derived immortalized canine smooth muscle cells
• Experiments requiring α-smooth muscle actin-characterized canine cells
• Research requiring extended expansion for more than 10 passages under the specified culture conditions
• Comparative cell research requiring a beagle small intestinal tissue source

The product documentation does not specify validated disease models, functional contraction assays, drug-screening applications, or therapeutic applications.

Culture Requirements

Recommended Medium

The suggested medium is M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 mL. The cells are documented as being grown in gelatin pre-coated tissue culture flasks with Complete Growth Medium.

Culture Surface

Use gelatin pre-coated tissue culture flasks under the cell culture conditions specified in the applicable culture protocol.

Expansion

• Cells can be expanded for more than 10 passages under the specified cell culture conditions.
• The recommended split ratio is 1:2.
• Repeated freezing and thawing of the cells are not recommended.

Detailed thawing, medium-change, centrifugation, incubation, and subculture procedures are not provided in the attached product datasheet. Follow the applicable supplier cell culture protocol when handling the cells.

Quality Control

Shipping and Storage

• Cryopreserved cells are shipped on dry ice using overnight delivery.
• Upon arrival, immediately transfer the frozen vial to liquid nitrogen storage at -180°C until ready for use.
• Do not keep the cryopreserved cells in a -20°C or -80°C freezer.
• Live cell shipment is available on request.
• Repeated freezing and thawing are not recommended.

Handling and Authorized Use

• Handle the cells with caution and treat all animal cells as potential pathogens.
• No testing procedure can completely guarantee the absence of infectious agents.
• The cells are distributed for research purposes only.
• The product is not authorized for human use, in vitro diagnostic procedures, or therapeutic procedures.
• Transfer or resale requires the supplier's written consent and remains subject to the applicable terms and conditions.

Warranty Conditions

The documented cell guarantee applies when the cells are purchased and used with the specified cell culture medium and coating solution, cultured and tested according to the applicable online protocols, and evaluated within 35 days from the date of product delivery.

Documents

D-6088IM.RFP

Frequently Asked Questions