Immortalized RFP-Expressing Canine Primary Coronary Artery Smooth Muscle Cells

Description

Research involving canine vascular biology often requires a reproducible smooth muscle cell model that maintains tissue relevance while supporting consistent cell availability for repeated experiments. Conventional primary cells may have limited expansion capability, creating challenges for long-term in vitro studies and fluorescence-based cell observation.

Immortalized RFP-expressing canine coronary artery smooth muscle cells provide a fluorescent canine vascular smooth muscle cell model derived from coronary artery tissue of beagle dogs. These cells are immortalized from primary cells through over-expression of the SV40 large T-antigen or human telomerase reverse transcriptase (hTERT), providing an extended lifespan cell model for research workflows requiring expanded cell availability.

The RFP-expressing canine smooth muscle cells are developed through RFP-lentiviral particle transduction. Red fluorescent protein generates bright red fluorescence when exposed to yellow-green light, enabling visual identification and fluorescence-based observation of canine coronary artery smooth muscle cells in research applications.

Cells are supplied cryopreserved at passage 2–3 and characterized by immunofluorescence staining with α-smooth muscle actin antibody. The cells are tested negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages under the recommended culture conditions.

Specifications

Features

• Immortalized RFP-expressing canine coronary artery smooth muscle cells

• Derived from coronary artery tissue of beagle dogs

• RFP expression enables fluorescent identification and cell observation workflows

• Generated through RFP-lentiviral particle transduction

• Immortalized through SV40 large T-antigen or hTERT over-expression

• Characterized by α-smooth muscle actin immunofluorescence staining

• Cryopreserved cells supplied at passage 2–3

• Expandable for more than 10 passages under recommended culture conditions

• Tested negative for bacteria, yeast, fungi, and mycoplasma

• Research use only (RUO)

Applications

• Canine Coronary Artery Smooth Muscle Cell Research

  Provides a canine coronary artery smooth muscle cell model derived from beagle dog tissue for in vitro vascular smooth muscle research.

• Fluorescence-Based Cell Tracking

  RFP expression provides a visible fluorescence signal that supports identification and observation of canine smooth muscle cells in fluorescence-based workflows.

• Canine Vascular Biology Studies

  Supports research involving canine vascular smooth muscle cell behavior, culture systems, and tissue-derived vascular models.

• Immortalized Smooth Muscle Cell Culture Models

  The extended lifespan characteristics of immortalized cells support research requiring repeated cell expansion and consistent cell availability.

• Fluorescence Imaging Workflows

  RFP-expressing cells provide a fluorescent marker for visualization under appropriate imaging conditions.

• Cell Characterization Studies

  Suitable for workflows involving smooth muscle cell identification using α-smooth muscle actin immunofluorescence characterization.

Storage and Handling

• Cryopreserved cells are shipped with dry ice overnight.

• Transfer frozen cells to liquid nitrogen storage (-180°C) immediately after arrival.

• Primary cells should not be stored long-term at -20°C or -80°C freezer temperatures.

• Repeated freezing and thawing of cells is not recommended.

• Animal-derived cells should be handled with appropriate laboratory safety precautions.

• For research use only. Not intended for human use, diagnostic procedures, or therapeutic applications.

Quality Control

Documents

D-6225IM.RFP

Frequently Asked Questions