Immortalized RFP-Expressing Canine Primary Colonic Smooth Muscle Cells

Description

When extended cell culture and direct fluorescence observation are required, conventional primary cells may not provide the lifespan or existing fluorescent signal needed for the planned workflow. Immortalized RFP-Expressing Canine Primary Colonic Smooth Muscle Cells provide a primary-derived, extended-lifespan canine colonic smooth muscle cell model with red fluorescent protein expression.

These cells are isolated from the colonic tissue of beagle dogs and cultured in gelatin pre-coated tissue culture flasks with Cell Biologics' Complete Growth Medium. Cells at passages 2–3 are harvested and cryopreserved in vials. Each vial contains 0.5 × 10⁶ cells per mL and is delivered frozen.

The canine colonic smooth muscle cells are characterized by immunofluorescence staining with an α-smooth muscle actin antibody. Testing is reported negative for bacteria, yeast, fungi, and mycoplasma.

RFP-expressing cells are developed through transduction with RFP-lentiviral particles. Red fluorescent protein produces bright red fluorescence when exposed to yellow-green light.

Under the cell culture conditions specified by Cell Biologics, these immortalized RFP-expressing canine colonic smooth muscle cells can be expanded for more than 10 passages at a split ratio of 1:2. Repeated freezing and thawing is not recommended.

Specifications

Features

• Primary-derived immortalized smooth muscle cells isolated from beagle dog colonic tissue
• RFP expression introduced through transduction with RFP-lentiviral particles
• Cryopreserved at passages 2–3 and supplied as a frozen vial
• Characterized by α-smooth muscle actin immunofluorescence staining
• Reported negative for bacteria, yeast, fungi, and mycoplasma
• Expandable for more than 10 passages under the specified culture conditions
• Recommended split ratio of 1:2
• Cultured in gelatin pre-coated tissue culture flasks
• Suggested medium: M2268 Complete Smooth Muscle Cell Medium w/ Kit
• Shipped overnight on dry ice for immediate transfer to liquid nitrogen

Applications

Immortalized RFP-expressing canine colonic smooth muscle cells may be considered for research workflows requiring a canine colon-derived smooth muscle cell model with an existing red fluorescent signal and extended culture capability.

• Fluorescence-based observation of RFP-expressing cells
• Extended in vitro culture using primary-derived immortalized canine cells
• Canine colonic smooth muscle cell culture research
• Comparative cell culture studies requiring a defined canine colonic tissue source
• Research workflows requiring α-smooth muscle actin-characterized cells

Researchers should confirm that the documented product characteristics and manufacturer-specified culture conditions are appropriate for their experimental design. No application-specific performance claims are provided in the supplied datasheet.

Characterization and Quality Control

Culture Requirements

• Use gelatin pre-coated tissue culture flasks.
• The suggested medium is M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 mL.
• Use a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics.
• The cells can be expanded for more than 10 passages under the specified conditions.
• Avoid repeated freezing and thawing.

The supplied datasheet does not provide detailed thawing, incubation, feeding, or subculture procedures. Follow the applicable Cell Biologics online protocol before use.

Shipping and Storage

• Cryopreserved cells are shipped overnight on dry ice.
• Immediately transfer the frozen vial to liquid nitrogen (-180°C) upon arrival.
• Do not store the frozen cells in a -20°C or -80°C freezer.
• Keep the cells in liquid nitrogen until ready for use.
• Live cell shipment is available on request.
• Repeated freezing and thawing is not recommended.

Research Use and Handling

This product is distributed for research purposes only. It is not authorized for human use, in vitro diagnostic procedures, or therapeutic procedures.

Investigators should handle the cells with caution and treat all animal cells as potential pathogens because no testing procedure can completely guarantee the absence of infectious agents.

Transfer or resale of Cell Biologics cells or products to other markets, organizations, or individuals is prohibited without written consent from Cell Biologics. Cell Biologics' Terms and Conditions must be accepted before submitting an order.

Warranty Information

According to the manufacturer, the guarantee applies only when Cell Biologics cells are purchased and used with Cell Biologics media and coating solution, appropriate cell culture and cell testing are performed according to Cell Biologics' online protocols, and the applicable work is completed within 35 days of product delivery.

Documents

D-6001IM.RFP

Frequently Asked Questions