Immortalized RFP-Expressing Canine Primary Artery Smooth Muscle Cells

Description

Research involving fluorescently labeled vascular smooth muscle cells requires a model that combines an identifiable fluorescent signal with an extended culture lifespan. These immortalized RFP-expressing canine primary artery smooth muscle cells are isolated from the artery tissue of beagle dogs and grown in gelatin pre-coated tissue culture flasks with Complete Growth Medium.

Red fluorescent protein (RFP) produces bright red fluorescence when exposed to yellow-green light. The stable RFP-expressing cells are developed through transduction with RFP-lentiviral particles, providing fluorescently labeled canine artery smooth muscle cells for research use.

Cells are harvested at passages 2–3 and cryopreserved in vials. Each vial contains 0.5 × 10⁶ cells per mL and is delivered frozen. The cells are characterized by immunofluorescence staining with an α-smooth muscle actin antibody and are reported negative for bacteria, yeast, fungi, and mycoplasma.

Under the specified cell culture conditions, these primary-cell-derived immortalized canine smooth muscle cells can be expanded for more than 10 passages at a split ratio of 1:2. Repeated freezing and thawing are not recommended.

Specifications

Features

• Derived from primary canine artery smooth muscle cells isolated from beagle dog artery tissue
• Stable RFP-expressing cells developed through transduction with RFP-lentiviral particles
• Produces bright red fluorescence when exposed to yellow-green light
• Harvested and cryopreserved at passages 2–3
• Supplied frozen at 0.5 × 10⁶ cells per mL
• Characterized by α-smooth muscle actin immunofluorescence staining
• Reported negative for bacteria, yeast, fungi, and mycoplasma
• Expandable for more than 10 passages at a split ratio of 1:2 under specified conditions
• Recommended for culture in gelatin pre-coated tissue culture flasks
• Shipped frozen on dry ice for immediate transfer to liquid nitrogen storage

RFP Expression and Immortalized Cell Format

RFP-expressing cells are developed through transduction with RFP-lentiviral particles. Red fluorescent protein, described as a mutant form of green fluorescent protein, produces bright red fluorescence when exposed to yellow-green light.

The immortalized cells are derived from primary cells with an extended lifespan. The product datasheet states that the associated primary-cell immortalization process uses overexpression of either the large T-antigen of simian virus 40 (SV40) or human telomerase reverse transcriptase (hTERT). The datasheet does not identify which of these two methods is used specifically for D-6081IM.RFP.

Applications

These immortalized RFP-expressing canine artery smooth muscle cells are intended for research requiring primary-cell-derived canine vascular smooth muscle cells with a stable red fluorescent signal and an extended culture lifespan.

• Research involving RFP-expressing canine artery smooth muscle cells
• Fluorescence-based observation of canine vascular smooth muscle cells
• Research requiring red fluorescent identification of cultured canine smooth muscle cells
• Studies requiring primary-cell-derived immortalized canine smooth muscle cells that can be expanded for more than 10 passages under the specified conditions

The product is for research use only and is not authorized for human use, in vitro diagnostic procedures, or therapeutic procedures.

Quality Control

Culture and Expansion

• Use M2268 Complete Smooth Muscle Cell Medium w/ Kit – 500 mL as the suggested medium.
• Grow cells in gelatin pre-coated tissue culture flasks with Complete Growth Medium.
• Under the specified culture conditions, cells can be expanded for more than 10 passages.
• Use a split ratio of 1:2 under the specified cell culture conditions.
• Repeated freezing and thawing of the cells are not recommended.

Shipping, Storage, and Handling

• Cryopreserved cells are shipped frozen with dry ice overnight.
• Upon arrival, immediately transfer the frozen cells to liquid nitrogen (-180°C) until ready for use.
• Do not keep the cells in a -20°C or -80°C freezer for long-term storage.
• Live cell shipment is available on request.
• Avoid repeated freezing and thawing.
• Handle the cells with caution and treat all animal cells as potential pathogens because no testing procedure can completely guarantee the absence of infectious agents.

Documents

D-6081IM.RFP Product Datasheet

Frequently Asked Questions