Immortalized Rabbit Primary Mammary Epithelial Cells (0.5x10^6)

Description

In epithelial cell research, inconsistent primary mammary cell behavior, limited lifespan, and donor variability often lead to unreliable experimental outcomes in breast tissue modeling, barrier function studies, and drug screening assays. Researchers frequently require a stable and reproducible rabbit mammary epithelial cell model that maintains epithelial phenotype while enabling long-term culture and repeatable experimental design.

This immortalized rabbit mammary epithelial cell system is derived from primary breast epithelial tissue and stabilized using SV40 large T antigen or hTERT immortalization, providing extended proliferative capacity while preserving key epithelial characteristics such as tight junction formation (ZO-1 expression), cell polarity, and adhesion behavior.

Designed for in vitro epithelial modeling, mammary gland biology, and drug response analysis, this system supports reproducible culture performance across multiple passages and enables consistent experimental workflows in advanced cell biology and translational research.

Features

• Immortalized rabbit mammary epithelial cell model (SV40 / hTERT stabilized)
• Maintains epithelial phenotype and tight junction marker expression (ZO-1)
• Supports long-term expansion (>10 passages)
• Gelatin-coated surface optimized for epithelial attachment
• Compatible with standard epithelial growth medium systems
• Cryopreserved format for stable storage and transport

Specifications

Applications

• Mammary epithelial cell biology research

• Breast tissue in vitro modeling

• Epithelial barrier function studies

• Tight junction (ZO-1) analysis

• Cell signaling pathway research

• Drug screening and toxicity testing

• Epithelial differentiation studies

• 3D epithelial tissue modeling

Cell Culture System

Gelatin-coated culture surface is required to support epithelial adhesion and morphology stability.

• Coating: Gelatin-based extracellular matrix mimic

• Incubation: 2–5 minutes coating before seeding

• Purpose: Enhance epithelial attachment and polarity maintenance

Gelatin coating improves cell adhesion by providing collagen-derived attachment motifs rich in glycine and proline residues, supporting epithelial morphology stability.

Standard Workflow Overview

Cell Recovery

1. Rapid thaw at 37°C water bath

2. Centrifuge 200 × g for 5 min

3. Remove cryoprotectant (DMSO)

4. Resuspend in pre-warmed medium

Subculture

1. Trypsin-EDTA (0.25%) digestion 3–5 min

2. Neutralization with serum-containing medium

3. Split ratio: 1:2 at ~90% confluency

Freezing

1. Freeze medium: 10% DMSO + 50% FBS + 40% basal medium

2. Controlled cooling at -80°C (6–12 h)

3. Long-term storage in liquid nitrogen

Storage & Handling

• Store in liquid nitrogen (-180°C) only

• Do not store at -20°C or -80°C for long-term

• Avoid repeated freeze-thaw cycles

• Handle all animal-derived cells under biosafety conditions

Documents

N-6035IM

Cell-Culture-Protocol-CB

Frequently Asked Questions