Immortalized Porcine Primary Pancreatic Microvascular Endothelial Cells (0.5x10^6)

Description

Primary endothelial cells are valuable models for studying vascular biology, but limited expansion capacity and variability between primary cell preparations can create challenges for long-term experimental workflows. Researchers studying microvascular function, endothelial responses, and vascular-related mechanisms often require stable endothelial cell models that maintain relevant biological characteristics while supporting reproducible culture.

Immortalized porcine pancreatic microvascular endothelial cells provide a research model derived from porcine pancreatic tissue for investigating endothelial biology and microvascular processes. These cells are designed for studies involving endothelial function, vascular signaling, microvascular research, and in vitro vascular model development.

This immortalized endothelial cell model is characterized by DiI-Ac-LDL uptake, a functional marker associated with endothelial cells, and supports extended culture compared with conventional primary endothelial cell workflows. The cells are supplied as cryopreserved material with a standardized culture protocol for recovery, expansion, and storage.

Key Features

• Immortalized porcine primary pancreatic microvascular endothelial cell model

• Derived from pancreatic tissue of American Landrace pigs

• Extended culture capability compared with standard primary endothelial cells

• Characterized by fluorescence-labeled DiI-Ac-LDL uptake assay

• Compatible with endothelial cell culture workflows

• Cryopreserved format for research use

Technical Overview

Applications

• Microvascular Endothelial Research

  Study endothelial characteristics and microvascular biology using a porcine-derived endothelial model.

• Vascular Biology Studies

  Investigate endothelial responses, vascular signaling, and cellular behavior.

• Endothelial Function Analysis

  Support functional studies involving endothelial cell characteristics and uptake assays.

• Angiogenesis Research

  Provide an in vitro endothelial cell model for vascular development-related studies.

• Pancreatic Vascular Research

  Support research involving pancreatic microvascular environments and endothelial interactions.

• Drug Screening and Toxicity Studies

  Evaluate compound effects on endothelial cell behavior in laboratory research workflows.

Cell Culture Workflow

Culture Conditions

• Culture vessels should be pre-coated with gelatin-based coating solution before cell seeding.

• Maintain cells under recommended endothelial cell culture conditions.

• Use sterile handling procedures during all cell culture operations.

Cell Recovery from Cryovial

1. Rapidly thaw frozen cells at 37°C until only small ice crystals remain.

2. Transfer cells into pre-warmed culture medium.

3. Centrifuge to remove residual cryoprotectant.

4. Seed cells into gelatin-coated culture vessels.

5. Maintain cells in a humidified 37°C, 5% CO₂ incubator.

Cell Expansion

• Wash cells with PBS before passaging.

• Detach cells using 0.25% Trypsin-EDTA solution.

• Recommended split ratio: 1:2.

• Monitor cell morphology and confluency regularly.

Storage and Handling

• Cryopreserved cells should be transferred to liquid nitrogen storage after receipt.

• Do not store cells long-term at -20°C or -80°C.

• Avoid repeated freeze-thaw cycles.

• Handle animal-derived cells using appropriate biosafety procedures.

Quality Control

• Tested negative for bacteria.

• Tested negative for yeast.

• Tested negative for fungi.

• Tested negative for mycoplasma.

Documents

P-6206IM

Cell-Culture-Protocol-CB

Frequently Asked Questions