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Description
Primary human sebocytes are difficult to obtain and lose proliferative capacity after a few passages, which limits their use in sebum, acne and skin barrier research. Biofargo Immortalized Human Sebaceous Gland Cells provide a stable, expandable human sebocyte model for in vitro studies.
The cells were derived from the sebaceous gland of a 30-year-old male donor and immortalized by lentiviral transduction of the SV40 large T antigen. They grow as adherent polygonal cells on gelatin-coated vessels in epithelial cell medium and can be subcultured at 1:3 to 1:5.
Specifications
| Item | Specifications |
|---|---|
| Catalog Number | BFCL0142 |
| Species | Human (Homo sapiens) |
| Tissue Source | Sebaceous gland |
| Disease | Unknown |
| Donor Age / Sex | 30 years / Male |
| Morphology | Polygonal |
| Growth Properties | Adherent |
| Immortalization | SV40 large T antigen, lentiviral transduction |
| Culture Medium | Epithelial cell complete medium |
| Culture Conditions | 37 °C, 5% CO2; 0.1% gelatin-coated vessels |
| Subculture Ratio | 1:3 to 1:5 |
| Medium Change | Half-medium change every other day |
| Biosafety Level | BSL-1 |
Features
- Human sebocyte origin: Derived from human sebaceous gland tissue from a defined adult donor.
- Extended proliferation: SV40 large T antigen immortalization allows continuous expansion beyond the limited lifespan of primary sebocytes.
- Adherent epithelial growth: Polygonal adherent cells grown on gelatin-coated vessels with standard trypsin–EDTA passaging.
- BSL-1 handling: Suitable for routine cell culture laboratories.
Applications
- Sebum production and lipid metabolism studies.
- Acne and sebaceous gland disorder models.
- Screening of dermatological and cosmetic active ingredients.
- Androgen, retinoid and hormone signaling in sebocytes.
- Skin inflammation and barrier function research.
Kit Components
- Immortalized Human Sebaceous Gland Cells, 1 cryovial
- Product datasheet
Storage & Shipping
- Shipped frozen.
- Store in liquid nitrogen. Storage at −80 °C reduces viability. Thaw and culture immediately upon receipt.
FAQs
What medium should be used for these cells?
Use epithelial cell complete medium. Culture at 37 °C with 5% CO2.
Do the culture vessels need coating?
Yes. Coat vessels with 0.1% gelatin at 37 °C for 1 h and wash once with PBS before seeding.
How were the cells immortalized?
The cells were immortalized by lentiviral transduction of the SV40 large T antigen.
How should the cryovial be thawed?
Thaw quickly in a 37 °C water bath for about 2 minutes, transfer to 5 mL complete medium, centrifuge at 200 × g for 5–10 min to remove the freezing medium, and seed into a gelatin-coated flask.
What subculture ratio is recommended?
Subculture at 1:3 to 1:5 by flask surface area using 0.25% trypsin–0.53 mM EDTA.
