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Human IL-10, h-IL-10, rh-IL-10, recombinant IL-10, interleukin-11
$198.00
Sku: RP300-10UG
Bcakground
Interleukin 10 ,also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includesIL-19, IL-20, IL-22, IL-24, and IL-26/AK155 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature human IL-10 shares 72%-86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL-20 R alpha, IL-22R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29(11-13). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14-16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).
Reference
1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
2. Sabat, R. et al. (2010) Cytokine Growth Factor Rev. 21:331.
3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981
4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
7. Hsu, D.-H. et al. (1990) Science 250:830.
8. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
9. Syto, R. et al. (1998) Biochemistry 37:16943.
10. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
11. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
12. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
13. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
14. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
15. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
16. Blackburn, S.D. and E.J. Wherry (2007)Trends Microbiol. 15:143.
Specifications
| Synonyms | CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A |
| Accession # | P22301 |
| Source | Human embryonic kidney cell, HEK293-derived human IL-10 protein |
| Ser19-Asn178 | |
| Predicted Moleucular weight | 18.6 kDa |
Components and Storage
| Formulation | Solution protein. |
| Dissolved in sterile PBS buffer. | |
| This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening. | |
| Storage and Stability | Avoid repeated freeze-thaw cycles. |
| It is recommended that the protein be aliquoted for optimal storage. | |
| 12 months from date of receipt, -20 to -70 °C as supplied. | |
| Shipping | Shipping with dry ice. |
Quality
| Purity | > 95%, determined by SDS-PAGE. |
| Endotoxin Level | <0.010 EU per 1 ug of the protein by the LAL method. |
| Activity | Measured in a cell proliferation assay using MC/9-2 mouse mast cells. |
| The EC50 for this effect is 50-150 pg/mL. |
SDS-PAGE
Gel filtration
Bioactivity
Documents
When can I expect my order to ship?
Most orders are filled and shipped within 2-3 business days from the time they are received.
Our standard shipping usually take 2-5 days.
We also provide express shippping for time-sensitive deliveries.
Email contact@biofargo.com if you have any requirements.
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